Biomaterials in Dentistry Research Group, Department of Stomatology, School of Dentistry, University of Granada, Spain; Medicina Clínica y Salud Pública PhD Programme, Spain.
Biomedical Group (BIO277), Department of Stomatology, School of Dentistry, University of Granada, Spain; Instituto Investigación Biosanitaria, ibs, Granada, Granada, Spain.
J Dent. 2021 Jun;109:103676. doi: 10.1016/j.jdent.2021.103676. Epub 2021 Apr 20.
To analyze how novel developed silicon dioxide composite membranes, functionalized with zinc or doxycycline, can modulate the expression of genes related to the osteogenic functional capacity of osteoblastic cells.
The composite nanofibers membranes were manufactured by using a novel polymeric blend and 20 nm silicon dioxide nanoparticles (SiO-NPs). To manufacture the membranes, 20 nm SiO-NPs were added to the polymer solution and the resulting suspension was processed by electrospinning. In a second step, the membranes were functionalized with zinc or doxycycline. Then, they were subjected to MG63 osteoblast-like cells culturing for 48 h. After this time, real-time quantitative polymerase chain reaction (RT-qPCR) was carried out to study the expression of Runx-2, OSX, ALP, OSC, OPG, RANKL, Col-I, BMP-2, BMP-7, TGF-β1, VEGF, TGF-βR1, TGF- βR2, and TGF-βR3. Mean comparisons were conducted by One-way ANOVA and Tukey tests (p < 0.05).
In general, the blending of SiO-NPs in the tested non-resorbable polymeric scaffold improves the expression of osteogenic genes over the control membranes. Doxycycline doping of experimental scaffolds attained the best results, encountering up-regulation of BMP-2, ALP, OPG, TGFβ-1 and TGFβ-R1. Membranes with zinc induced a significant increase in the expression of Col-I, ALP and TGF β1. Both, zinc and doxycycline functionalized membranes enormously down-regulated the expression of RANKL.
Zinc and doxycycline doped membranes are bioactive inducing overexpression of several osteogenic gene markers.
Doxycycline doped membranes may be a potential candidate for use in GBR procedures in several challenging pathologies, including periodontal diseases.
分析新型合成二氧化硅复合膜（经锌或强力霉素功能化）如何调节成骨细胞中与成骨功能能力相关基因的表达。
采用新型聚合物共混物和 20nm 二氧化硅纳米粒子（SiO-NPs）制造复合纳米纤维膜。为了制造膜，将 20nmSiO-NPs 添加到聚合物溶液中，然后通过静电纺丝处理得到悬浮液。在第二步中，将膜用锌或强力霉素功能化。然后，将其用于 MG63 成骨样细胞培养 48 小时。48 小时后，进行实时定量聚合酶链反应（RT-qPCR）以研究 Runx-2、OSX、ALP、OSC、OPG、RANKL、Col-I、BMP-2、BMP-7、TGF-β1、VEGF、TGF-βR1、TGF-βR2 和 TGF-βR3 的表达。通过单向方差分析和 Tukey 检验进行均值比较（p<0.05）。
一般来说，在测试的不可吸收聚合物支架中混合 SiO-NPs 可改善对照膜中成骨基因的表达。实验支架中强力霉素的掺杂获得了最佳结果，可上调 BMP-2、ALP、OPG、TGFβ-1 和 TGFβ-R1 的表达。含锌的膜诱导 Col-I、ALP 和 TGFβ-1 的表达显著增加。锌和强力霉素功能化的膜都极大地下调了 RANKL 的表达。
锌和强力霉素掺杂的膜具有生物活性，可诱导多种成骨基因标志物的过表达。
强力霉素掺杂的膜可能是在包括牙周病在内的几种具有挑战性的病理情况下，用于 GBR 程序的潜在候选物。
