Medical Biophysics Division, Physics Department, Faculty of Science, Helwan University, Cairo, Egypt.
Biophysics Department, Faculty of Science, Cairo University, Giza, Egypt.
Biochim Biophys Acta Mol Basis Dis. 2021 Jul 1;1867(7):166150. doi: 10.1016/j.bbadis.2021.166150. Epub 2021 Apr 20.
The present study investigated the efficacy of cationic liposome-encapsulated carotenoids (lutein or beta-carotene) as a treatment in an animal model of fibromyalgia (FM). Preparation and characterization of the nano-sized cationic liposomal carotenoids have been carried out. FM has been induced in the experimental animals via successive subcutaneous reserpine injection (1 mg/kg). Animals were divided into four groups; control, reserpinized (Res), reserpinized and cationic liposomal lutein-treated (Res + CL-Lut), and reserpinized and liposomal beta-carotene-treated (Res + CL-Bc). Levels of norepinephrine (NE), dopamine (DA), and serotonin (5-HT), and oxidative stress markers (MDA, HO, NO, and GSH) were determined in the brain's cortical tissue of the different groups of animals. Furthermore, the spectral analysis of the electrocorticogram (ECoG) was carried out. Animal behavior was tested for different animal groups. Results showed a significant reduction in monoamines, an elevation of oxidative stress markers, a shift in the ECoG frequency band power, and a change in pain threshold of the reserpinized animals. A return to a non-significant difference from the control values of all the measured parameters has been obtained after two weeks of cationic liposomal carotenoid preparations treatment. The present findings shed more light on the validity of the reserpine model of FM and provide evidence for the antidepressant, antioxidant, and anti-nociceptive potential of the cationic liposomal carotenoids. The present results proofed that the natural product preparations on a nano-sized scale could be a good alternative to the pharmacological interventions for FM treatment.
本研究探讨了阳离子脂质体包裹类胡萝卜素（叶黄素或β-胡萝卜素）作为纤维肌痛（FM）动物模型治疗方法的疗效。已经进行了纳米级阳离子脂质体类胡萝卜素的制备和特性研究。通过连续皮下给予利血平（1mg/kg）在实验动物中诱导 FM。将动物分为四组; 对照组、利血平化（Res）、利血平化和阳离子脂质体叶黄素处理（Res+CL-Lut）以及利血平化和脂质体β-胡萝卜素处理（Res+CL-Bc）。测定不同组动物大脑皮质组织中的去甲肾上腺素（NE）、多巴胺（DA）和 5-羟色胺（5-HT）水平以及氧化应激标志物（MDA、HO、NO 和 GSH）。此外，还进行了脑电图（ECoG）的光谱分析。对不同动物组的动物行为进行了测试。结果显示，单胺类物质显著减少，氧化应激标志物升高，ECoG 频带功率发生转移，利血平化动物的疼痛阈值发生变化。经过两周的阳离子脂质体类胡萝卜素制剂治疗后，所有测量参数均恢复到与对照组无显著差异的水平。本研究结果进一步证实了利血平 FM 模型的有效性，并为阳离子脂质体类胡萝卜素的抗抑郁、抗氧化和抗伤害作用提供了证据。本研究结果证明，在纳米级规模上的天然产物制剂可能是 FM 治疗的药理学干预的良好替代方法。
