Department of Physical Electronics, Faculty of Science, Masaryk University, Kotlarska 267/2, 611 37 Brno, Czech Republic; Atomic and Molecular Physics Department, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran.
Department of Physics, Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz-Maragheh Road, 53714-161 Tabriz, Iran.
Bioorg Chem. 2021 Jun;111:104892. doi: 10.1016/j.bioorg.2021.104892. Epub 2021 Apr 7.
Cervical cancer is one of the important cancers in women. Research on novel treatment approach can reduce the mortality and burden. Although radiotherapy is a common treatment, its negative side effects have concerned physician. In our study, we studied impact of cold atmospheric pressure plasma on the Hela cancer cells, as an alternative treatment. The effect of three different types of such plasma; dielectric barrier discharge (DBD), plasma jet, and afterglow plasma, on the cancer cells were studied. Moreover, some effective operating parameters such as exposure time, applied voltage, composition of working gas in plasma treatment were investigated on the survival of the afterglow plasma. Finally, treatments by the afterglow plasma, gamma radiation (1 Gy), and combination of both were compared. Analysis showed that DBD and plasma jet (direct exposure) effectively killed the cancer cells, even by a minimum applied voltage. But a fraction of the cells survived after the exposure of indirect diffused afterglow plasma. In the case of this plasma, we realized that higher applied voltage and exposure time led to less cell viability. Fewer fractions of survival cells were detected in the case of argon afterglow plasma comparing to oxygen afterglow. Cold atmospheric plasma and its combination with radiation therapy showed a significant decrease in viability of the cells, comparing to the radiation alone. Our research showed that plasma and its combination with radiation therapy have superiority over radiation therapy.
宫颈癌是女性癌症中的重要癌症之一。研究新的治疗方法可以降低死亡率和负担。虽然放疗是一种常见的治疗方法，但它的副作用引起了医生的关注。在我们的研究中，我们研究了冷大气压等离子体对 Hela 癌细胞的影响，作为一种替代治疗方法。研究了三种不同类型的等离子体；介质阻挡放电（DBD）、等离子射流和余晖等离子体对癌细胞的影响。此外，还研究了不同的有效操作参数，如暴露时间、施加电压、等离子体处理中工作气体的组成，对余晖等离子体存活的影响。最后，比较了余晖等离子体、γ 辐射（1 Gy）和两者联合治疗的效果。分析表明，DBD 和等离子射流（直接暴露）能有效杀死癌细胞，即使施加的电压很低。但是，间接扩散的余晖等离子体暴露后仍有一部分细胞存活。在后一种情况下，我们发现施加的电压和暴露时间越高，细胞活力越低。与氧气余晖等离子体相比，氩气余晖等离子体的存活细胞分数较少。与单独放疗相比，冷大气压等离子体及其与放疗的联合治疗显著降低了细胞活力。我们的研究表明，等离子体及其与放疗的联合治疗优于单独放疗。
