SOS色变试验，一种基于细胞对遗传毒性剂的初级反应的比色测定法。

The SOS Chromotest, a colorimetric assay based on the primary cellular responses to genotoxic agents.

作者信息

Hofnung M, Quillardet P

机构信息

Unité de Programmation Moléculaire et Toxicologie Génétique, CNRS UA 271-INSERM U.163, Institut Pasteur, Paris, France.

出版信息

Ann N Y Acad Sci. 1988;534:817-25. doi: 10.1111/j.1749-6632.1988.tb30169.x.

DOI:10.1111/j.1749-6632.1988.tb30169.x

PMID:3389688

Abstract

The SOS Chromotest is a quantitative bacterial colorimetric assay for genotoxins. Substantial validation is now available. We describe the tester strain as well as results of validation studies. Comparison of the results with those obtained in the Mutatest (the Ames test) showed that most (90 percent) of the mutagenic compounds were also SOS inducers. For these compounds, a quantitative correlation was observed between the mutagenic potency and the SOS-inducing potency (SOSIP). The present data indicate that the SOS Chromotest has many practical advantages and may be used as a primary screening tool or as part of a battery of short-term tests for carcinogens. Theoretical considerations on the relation between the SOSIP and the mutagenic potency are briefly discussed.

摘要

SOS比色法是一种用于检测基因毒素的定量细菌比色测定法。目前已有大量验证数据。我们描述了测试菌株以及验证研究的结果。将这些结果与在Mutatest（艾姆斯试验）中获得的结果进行比较，发现大多数（90%）诱变化合物也是SOS诱导剂。对于这些化合物，观察到诱变效力与SOS诱导效力（SOSIP）之间存在定量相关性。目前的数据表明，SOS比色法具有许多实际优势，可作为主要筛选工具或作为致癌物短期试验组合的一部分。本文简要讨论了SOSIP与诱变效力之间关系的理论思考。