Quillardet P, Hofnung M
Mutat Res. 1985 Jun;147(3):65-78. doi: 10.1016/0165-1161(85)90020-2.
The SOS Chromotest is a quantitative bacterial colorimetric assay for genotoxins. Substantial validation is now available (Quillardet et al., 1985). We describe here in detail the tester strain as well as the effects of the variation of some parameters on the assay. We report a simple spot-test procedure as well as a new standard procedure which incorporate recent technical improvements aimed at simplifying the assay further.
SOS色变试验是一种用于检测基因毒素的定量细菌比色测定法。目前已有大量的验证研究(Quillardet等人,1985年)。我们在此详细描述测试菌株以及某些参数变化对该测定法的影响。我们报告了一种简单的斑点试验程序以及一种新的标准程序,该标准程序纳入了旨在进一步简化该测定法的最新技术改进。