Abou-Elela Dalia H, El-Edel Rawhia H, Shalaby Amr S, Fouaad Mariam A, Sonbol Ahmed A
Department of Clinical Pathology, Faculty of Medicine, Menoufia University, Shebin El Kom, Menoufia, Egypt.
Department of Neuro-Psychiatry, Faculty of Medicine, Menoufia University, Shebin El Kom, Menoufia, Egypt.
Indian J Psychiatry. 2020 Nov-Dec;62(6):678-683. doi: 10.4103/psychiatry.IndianJPsychiatry_783_19. Epub 2020 Dec 12.
Becoming shorter by each cell division, telomere length (TL) is regarded as a marker of cellular aging. Relative TL (T/S) depends on the quantitation of telomere hexamer repeat copy number normalized to autosomal single-copy gene copy number. TL is influenced by several factors, including oxidative stress (OS) and inflammation. This study aimed to investigate the possible role of TL and OS as markers for Alzheimer's disease (AD).
One hundred and eighty participants were categorized into three groups. Group 1: Included 60 patients with AD. Group II: included 60 age-matched nondemented subjects. Group III (pregeriatric group): included 60 healthy controls with their ages ranging between 30 and 60 years. TL was determined by the quantitative Real time-PCR method, plasma levels of 8-OHdG by enzyme-linked immunosorbent assay, and total antioxidant capacity (TAC) by colorimetery.
In comparison to the other two groups, patients with AD showed shortened TL, increased plasma 8-OHdG concentration, and decreased TAC. The sensitivity of T/S ratio to predict AD was 86.67%, whereas the specificity was 96.67%. The sensitivity of 8-OHdG to predict AD was 96.67%, whereas the specificity was 86.67%.
AD is associated with shortened TL and increased OS as manifested by decreased TAC and increased serum 8-OHdG. T/S and 8-OHdG could be used as early predictors for AD.
端粒长度（TL）会随着每次细胞分裂而缩短，被视为细胞衰老的标志物。相对端粒长度（T/S）取决于端粒六聚体重复拷贝数相对于常染色体单拷贝基因拷贝数的定量。端粒长度受多种因素影响，包括氧化应激（OS）和炎症。本研究旨在探讨端粒长度和氧化应激作为阿尔茨海默病（AD）标志物的可能作用。
180名参与者被分为三组。第一组：包括60例AD患者。第二组：包括60名年龄匹配的非痴呆受试者。第三组（老年前期组）：包括60名年龄在30至60岁之间的健康对照者。通过定量实时聚合酶链反应方法测定端粒长度，采用酶联免疫吸附测定法测定血浆8-羟基脱氧鸟苷水平，通过比色法测定总抗氧化能力（TAC）。
与其他两组相比，AD患者的端粒长度缩短，血浆8-羟基脱氧鸟苷浓度升高，总抗氧化能力降低。T/S比值预测AD的敏感性为86.67%，特异性为96.67%。8-羟基脱氧鸟苷预测AD的敏感性为96.67%，特异性为86.67%。
AD与端粒长度缩短和氧化应激增加有关，表现为总抗氧化能力降低和血清8-羟基脱氧鸟苷增加。T/S和8-羟基脱氧鸟苷可作为AD的早期预测指标。
