Klein H W, Gourley I M
Division of Plastic and Reconstructive Surgery, University of California, Davis, Sacramento 95817.
Ann Plast Surg. 1988 Jun;20(6):547-51. doi: 10.1097/00000637-198806000-00008.
The assessment of events that occur in elevated skin flaps has been largely by indirect methods. A method was sought that gives direct, reproducible, and accurate data about physiological and biochemical changes that occur during flap elevation and during periods of altered blood flow. Because of its ability to monitor changes in the levels of high energy phosphorus metabolites (ATP or adenosine triphosphate, PCr, or phosphocreatin, Pi, or inorganic phosphate), 31p magnetic resonance spectroscopy (MRS) holds promise of providing direct assessment of the metabolic status and biochemical changes that occur during skin flap elevation. MRS monitoring was performed on raised abdominal skin flaps of 12 rats. Abdominal flaps in 4 animals served as controls with and without total vascular occlusion while arterial blood flow was manipulated in 4 flaps and venous flow in 4 flaps. The results have validated the ability of MRS to determine cellular levels of ATP, PCr, and Pi in skin flaps, and to measure intracellular pH through the chemical shift of the Pi resonance.
对抬高皮瓣中发生的事件的评估主要采用间接方法。人们一直在寻找一种能提供关于皮瓣抬高期间和血流改变期间发生的生理和生化变化的直接、可重复且准确数据的方法。由于能够监测高能磷代谢物（ATP或三磷酸腺苷、PCr或磷酸肌酸、Pi或无机磷酸盐）水平的变化，31磷磁共振波谱（MRS）有望直接评估皮瓣抬高期间的代谢状态和生化变化。对12只大鼠的腹部抬高皮瓣进行了MRS监测。4只动物的腹部皮瓣作为有或无完全血管闭塞的对照，同时对4个皮瓣的动脉血流和4个皮瓣的静脉血流进行了控制。结果证实了MRS测定皮瓣中ATP、PCr和Pi细胞水平以及通过Pi共振的化学位移测量细胞内pH值的能力。
