Cheung A, Zhong J, Gore J C, Cuono C B
Department of Surgery, Yale University School of Medicine, New Haven, Connecticut.
Magn Reson Med. 1994 Nov;32(5):572-8. doi: 10.1002/mrm.1910320505.
We have used a rectangular surface coil and chemical shift imaging to conduct in vivo localized 31P NMR metabolic studies in a rat dorsal skin flap model. This approach permits regional comparisons without manipulation of either coil position or subject within the magnet bore. Both the PCr:Pi ratio (reflecting ischemia insult) and the PCr:ATP ratio (reflecting phosphagen reserves) decreased as functions of time and distance from the vascular pedicle. The maximum change was nearly 6-fold for the PCr:Pi ratio, and 3-fold for the PCr:ATP ratio. Signal contamination from subjacent muscle is constant and does not interfere with the metabolic evaluations of skin flaps. This technique may facilitate a better understanding of cutaneous metabolic derangements, such as burns and skin flaps used in reconstructive surgery, as well as studies of pharmacologic regimens developed for their treatment. It also holds potential for application in the study of congenital and neoplastic metabolic disorders of skin.
我们使用矩形表面线圈和化学位移成像技术，在大鼠背部皮瓣模型中进行体内局部31P NMR代谢研究。这种方法允许在不改变线圈位置或磁体腔内受试者位置的情况下进行区域比较。磷酸肌酸与无机磷酸的比值（反映缺血损伤）和磷酸肌酸与三磷酸腺苷的比值（反映磷酸原储备）均随时间以及与血管蒂距离的变化而降低。磷酸肌酸与无机磷酸的比值最大变化近6倍，磷酸肌酸与三磷酸腺苷的比值最大变化3倍。下方肌肉的信号污染是恒定的，不会干扰皮瓣的代谢评估。该技术可能有助于更好地理解皮肤代谢紊乱，如烧伤和重建手术中使用的皮瓣，以及为其治疗开发的药物方案的研究。它在皮肤先天性和肿瘤性代谢紊乱的研究中也具有应用潜力。
