急性低氧性高碳酸血症对内毒素血症兔全身中性粒细胞氧化活性的影响。
Effect of acute isooxic hypercapnia on oxidative activity of systemic neutrophils in endotoxemic rabbits.
作者信息
Billert Hanna, Bednarek Ewa, Kusza Krzysztof, Ponichter Mirosław, Kurpisz Maciej
机构信息
Department of Experimental Anaesthesiology, Chair of Anaesthesiology and Intensive Therapy, Poznan University of Medical Sciences, Poznan, Poland.
Clinics of Anaesthesiology and Intensive Therapy, Chair of Anaesthesiology and Intensive Therapy, Poznan University of Medical Sciences, Poznan, Poland.
出版信息
Cent Eur J Immunol. 2021;46(1):47-53. doi: 10.5114/ceji.2021.105245. Epub 2021 Apr 18.
INTRODUCTION
Whether carbon dioxide (CO) affects systemic oxidative phenomena under conditions of endotoxemia is not sufficiently clarified. The study aimed to assess the impact of moderate acute hypercapnia on the respiratory burst of circulating neutrophils in mechanically ventilated endotoxemic rabbits.
MATERIAL AND METHODS
Twenty-four endotoxemic rabbits were mechanically ventilated with standard or CO-enriched gas mixture in order to obtain isooxic hypercapnia. At a baseline point and following 180 min of hypercapnic ventilation, luminol-dependent chemiluminescence (CL) of circulating neutrophils and serum 2-thiobarbituric acid reactive substance (TBARS) concentrations were measured. Throughout the study, leukocyte and neutrophil counts, pH status, circulatory parameters and body temperature were also assessed.
RESULTS
Following 180 min of hypercapnic ventilation, opsonized zymosan (OZ)-stimulated neutrophils showed lower CL vs. the control group (p = 0.004). Other parameters studied were not affected.
CONCLUSIONS
Short-term isooxic hypercapnia in endotoxemic rabbits preserves circulating neutrophil count pattern and reactive oxygen species (ROS) generation, but it may reduce phagocytosis.
引言
内毒素血症情况下二氧化碳（CO₂）是否影响全身氧化现象尚未完全阐明。本研究旨在评估中度急性高碳酸血症对机械通气的内毒素血症兔循环中性粒细胞呼吸爆发的影响。
材料与方法
24只内毒素血症兔用标准或富含CO₂的气体混合物进行机械通气，以实现等氧高碳酸血症。在基线点和高碳酸血症通气180分钟后，测量循环中性粒细胞的鲁米诺依赖性化学发光（CL）和血清2-硫代巴比妥酸反应性物质（TBARS）浓度。在整个研究过程中，还评估了白细胞和中性粒细胞计数、pH状态、循环参数和体温。
结果
高碳酸血症通气180分钟后，调理酵母聚糖（OZ）刺激的中性粒细胞与对照组相比CL较低（p = 0.004）。研究的其他参数未受影响。
结论
内毒素血症兔短期等氧高碳酸血症可维持循环中性粒细胞计数模式和活性氧（ROS）生成，但可能会降低吞噬作用。
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