Zhao Wei, Luo Hongjie, Zhu Wanqing, Yuan Xiaoqin, Shao Jianchun
Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou, China.
Department of Ecology, Institute of Hydrobiology, College of Life Science and Technology, Jinan University, Guangzhou, China.
Front Physiol. 2021 Apr 9;12:661107. doi: 10.3389/fphys.2021.661107. eCollection 2021.
A 6-week feeding strategy experiment was conducted to investigate the effects of time-dependent protein restriction and subsequent recovery on shrimp. Diets with protein levels of 43 and 36% were used as adequate and restricted diets, respectively. Shrimp with an initial body weight of 6.52 ± 0.46 g were given four feeding strategies: feeding on an adequate diet for six weeks (T1, the control), having protein-restricted diet in weeks 1 and 4 (T2), being given a protein-restricted diet in weeks 1, 3, and 5 (T3), and having protein-restricted diet in weeks 1, 2, 4, and 5 (T4). WG, SGR, FE, and PER of shrimp in T1-T3 showed no significant difference ( > 0.05), these indicators of T4 were significantly reduced ( < 0.05). No significant differences were found in digestive enzyme activities of shrimp among all treatments ( > 0.05). Crude protein content of shrimp muscle in T4 was lower than that of T1-T3. The expression level of in T4 was lower than that in other treatments, while was higher than that of other treatments. To balance saving on feeding cost and growth performance, giving the shrimp a protein-restricted diet for 1 week with subsequent refeeding (T2 and T3) is suitable for shrimp under high-density conditions.
进行了一项为期6周的投喂策略实验，以研究随时间变化的蛋白质限制及随后的恢复对虾的影响。蛋白质水平分别为43%和36%的饲料分别用作充足饲料和限制饲料。初始体重为6.52±0.46克的虾被给予四种投喂策略：六周内投喂充足饲料（T1，对照组）、第1周和第4周投喂蛋白质限制饲料（T2）、第1周、第3周和第5周投喂蛋白质限制饲料（T3）、第1周、第2周、第4周和第5周投喂蛋白质限制饲料（T4）。T1 - T3组虾的增重率（WG）、特定生长率（SGR）、饲料效率（FE）和蛋白质效率（PER）无显著差异（>0.05），T4组的这些指标显著降低（<0.05）。各处理组虾的消化酶活性均无显著差异（>0.05）。T4组虾肌肉的粗蛋白含量低于T1 - T3组。T4组中[此处原文缺失具体基因或指标名称]的表达水平低于其他处理组，而[此处原文缺失具体基因或指标名称]高于其他处理组。为平衡饲料成本节约和生长性能，在高密度条件下，给虾投喂1周蛋白质限制饲料后再重新投喂（T2和T3）适合虾的养殖。
