Jenkins Sascha, Taylor Andrew, Jackson Alison C, Armitage Andrew D, Bates Helen J, Mead Andrew, Harrison Richard J, Clarkson John P
School of Life Sciences, Warwick Crop Centre, University of Warwick, Wellesbourne Campus, Warwick, United Kingdom.
NIAB-EMR, East Malling Research, Kent, United Kingdom.
Front Microbiol. 2021 Apr 9;12:593140. doi: 10.3389/fmicb.2021.593140. eCollection 2021.
is a soilborne fungal plant pathogen responsible for causing disease in many economically important crops with "special forms" () adapted to infect specific plant hosts. f. sp. (FOP) is the causal agent of Fusarium wilt disease of pea. It has been reported in every country where peas are grown commercially. Disease is generally controlled using resistant cultivars possessing single major gene resistance and therefore there is a constant risk of breakdown. The main aim of this work was to characterise isolates collected from diseased peas in the United Kingdom as well as FOP isolates obtained from other researchers representing different races through sequencing of a housekeeping gene and the presence of () genes, which have previously been associated with pathogenicity in other f. spp. isolates from diseased United Kingdom pea plants possessed none or just one or two known genes with no consistent pattern of presence/absence, leading to the conclusion that they were foot-rot causing isolates rather than FOP. In contrast, FOP isolates had different complements of genes with all those identified as race 1 containing , , , , , , , and . FOP isolates that were identified as belonging to race 2 through testing on differential pea cultivars, contained either , , , or , . Significant upregulation of genes was also observed over the early stages of infection by different FOP races in pea roots. Race specific gene profiling may therefore provide potential targets for molecular identification of FOP races but further research is needed to determine whether variation in complement of genes in FOP race 2 isolates results in differences in virulence across a broader set of pea differential cultivars.
是一种土壤传播的真菌性植物病原体,可导致许多具有经济重要性的作物发病,其“特殊形式”()适应于感染特定的植物宿主。豌豆尖镰孢菌(FOP)是豌豆枯萎病的病原体。在每个商业化种植豌豆的国家都有相关报道。通常使用具有单一主基因抗性的抗病品种来控制病害,因此始终存在抗性丧失的风险。这项工作的主要目的是通过对一个管家基因进行测序以及检测先前与其他尖镰孢菌物种致病性相关的()基因,来鉴定从英国患病豌豆中分离出的菌株以及从其他研究人员处获得的代表不同小种的FOP菌株。从英国患病豌豆植株中分离出的菌株没有或仅有一两个已知的基因,且不存在一致的有无模式,由此得出结论,它们是引起根腐病的菌株而非FOP。相比之下,FOP菌株具有不同的基因组合,所有被鉴定为小种1的菌株都含有、、、、、、、和。通过在鉴别豌豆品种上进行测试被鉴定为属于小种2的FOP菌株,含有、、、或。在豌豆根中,不同FOP小种在感染早期阶段也观察到基因的显著上调。因此,小种特异性基因谱分析可能为FOP小种的分子鉴定提供潜在靶点,但需要进一步研究以确定FOP小种2菌株中基因组合的差异是否会导致在更广泛的豌豆鉴别品种中致病力的差异。