Chakrapani Kota, Chanu W Tampakleima, Sinha Bireswar, Thangjam Bijeeta, Hasan Wajid, Devi Konjengbam Sarda, Chakma Tusi, Phurailatpam Sumitra, Mishra Lokesh Kumar, Singh Gopi Mohan, Khoyumthem Pramesh, Saini Rahul
Department of Plant Pathology, College of Agriculture, Central Agricultural University, Imphal, India.
KVK, Jahanabad, Bihar Agricultural University, Jahanabad, India.
Front Microbiol. 2023 Aug 3;14:1228442. doi: 10.3389/fmicb.2023.1228442. eCollection 2023.
Fusarium wilt caused by f. sp. () is an important disease and major obstacle to pea production, causing huge losses to growers. The focus of this study was on isolation followed by morphological, molecular characterization and analyzing the growth of the casual agent under variable temperature, pH and Nitrogen levels. The morphological features of radial growth, sporulation, pigmentation and mycelial characterization were examined and the variability of all isolates was presented. Molecular characterization of the fungus by ITS rDNA sequencing revealed that all 13 isolates belong to species. Six isolates were tested for temperature, pH and nitrogen dosage optimization studies. Seven different temperatures, viz., 21, 23, 25, 27, 29, 31, 33°C and pH values, having 3, 4, 5, 6, 7, 8, and 9 pH, as well as nitrogen dosage levels of 0 g, 3 g, 5 g, 7 g, 9 g, 11 g, and 13 g were tested against all six isolates, respectively. The results showed that all isolates exhibited the highest growth at a temperature of 25°C and the optimal temperature range for growth of was 23-27°C. All isolates showed the highest growth at pH5. Change in the nitrogen doses of the base ended in formation of thick, dense, fluffy mycelium of the casual agent. Six isolates were used for combination studies with seven different levels of temperatures, pH levels and nitrogen dosages. The density plots revealed the variations in the growth of the isolates with changes in temperature, pH and nitrogen levels, which can lead to mutations or genetic changes in the pathogens that could potentially introduce new threats to pea cultivation.
由尖孢镰刀菌（F. sp. ）引起的枯萎病是豌豆生产中的一种重要病害和主要障碍，给种植者造成了巨大损失。本研究的重点是分离病原菌，然后进行形态学、分子特征分析，并分析病原菌在不同温度、pH值和氮水平下的生长情况。研究了病原菌的径向生长、产孢、色素沉着和菌丝体特征等形态学特征，并展示了所有分离株的变异性。通过ITS rDNA测序对真菌进行分子特征分析，结果表明所有13个分离株均属于尖孢镰刀菌物种。对6个分离株进行了温度、pH值和氮用量优化研究。分别针对所有6个分离株测试了7种不同温度，即21、23、25、27、29、31、33°C，7种pH值，即3、4、5、6、7、8、9，以及0 g、3 g、5 g、7 g、9 g、11 g和13 g的氮用量水平。结果表明，所有分离株在25°C时生长最快，尖孢镰刀菌生长的最适温度范围为23 - 27°C。所有分离株在pH5时生长最快。改变基础氮剂量会导致病原菌形成浓密、厚实、蓬松的菌丝体。6个分离株用于7种不同温度、pH值水平和氮用量的组合研究。密度图揭示了随着温度、pH值和氮水平的变化，分离株生长的差异，这可能导致病原菌发生突变或基因变化，从而可能给豌豆种植带来新的威胁。
