Jiang Fufeng, Lei Tao, Wang Zhi, He Min, Zhang Jumei, Wang Juan, Zeng Haiyan, Chen Moutong, Xue Liang, Ye Qinghua, Pang Rui, Wu Shi, Gu Qihui, Ding Yu, Wu Qingping
School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an, China.
Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou, China.
Front Microbiol. 2021 Apr 9;12:656380. doi: 10.3389/fmicb.2021.656380. eCollection 2021.
is an important foodborne pathogen and its biofilm formation ability facilitates its colonization and persistence in foods by protecting it from stresses including environmental variation and antibiotic exposure. Several important proteins are involved in biofilm formation; however, the identity and function of many remain unknown. In this study, we discovered a hypothetical protein, VP0610 that negatively regulates biofilm formation in , and we found that the loss of typically results in pleiotropic phenotypes that contribute toward promoting biofilm formation, including significantly increased insoluble exopolysaccharide production and swimming motility, decreased soluble exopolysaccharide production, and decreased bis-(3'-5')-cyclic dimeric guanosine monophosphate production. Pull-down assays revealed that VP0610 can interact with 180 proteins, some of which (Hfq, VP0710, VP0793, and CyaA) participate in biofilm formation. Moreover, deleting enhanced the expression of genes responsible for biofilm component (), the sugar phosphotransferase system (PTS) EIIA component ( and ), and a high-density regulator of quorum sensing (), while reducing the expression of the bis-(3'-5')-cyclic dimeric guanosine monophosphate degradation protein (CdgC), resulting in faster biofilm formation. Taken together, our results indicate that is an integral member of the key biofilm regulatory network of that functions as a repressor of biofilm formation.
是一种重要的食源性病原体，其生物膜形成能力通过保护其免受包括环境变化和抗生素暴露在内的压力，促进其在食物中的定殖和持续存在。几种重要的蛋白质参与生物膜形成；然而，许多蛋白质的身份和功能仍然未知。在本研究中，我们发现了一种假定蛋白VP0610，它对生物膜形成具有负调控作用，并且我们发现其缺失通常会导致多效性表型，有助于促进生物膜形成，包括不溶性胞外多糖产量显著增加和游动性增强、可溶性胞外多糖产量降低以及双（3'-5'）-环二聚鸟苷酸产量降低。下拉实验表明，VP0610可以与180种蛋白质相互作用，其中一些（Hfq、VP0710、VP0793和CyaA）参与生物膜形成。此外，缺失增强了负责生物膜成分（）、糖磷酸转移酶系统（PTS）EIIA成分（和）以及群体感应的高密度调节因子（）的基因的表达，同时降低了双（3'-5'）-环二聚鸟苷酸降解蛋白（CdgC）的表达，导致生物膜形成更快。综上所述，我们的结果表明是生物膜关键调控网络的一个组成成员，作为生物膜形成的抑制因子发挥作用。
