通过全细胞斑点印迹杂交用染色体DNA探针快速准确鉴定肠道拟杆菌属菌种。
Rapid and correct identification of intestinal Bacteroides spp. with chromosomal DNA probes by whole-cell dot blot hybridization.
作者信息
Morotomi M, Ohno T, Mutai M
机构信息
Yakult Central Institute for Microbiological Research, Tokyo, Japan.
出版信息
Appl Environ Microbiol. 1988 May;54(5):1158-62. doi: 10.1128/aem.54.5.1158-1162.1988.
Abstract
A dot blot hybridization procedure with 32P-labeled whole chromosomal DNA of the type strains as probes was developed as a rapid and simple method for identification of intestinal Bacteroides species. Bacterial cells were fixed onto membrane filters by slight suction, treated with 0.5 N NaOH, and hybridized with these probes. Of 65 Bacteroides strains isolated from 19 human fecal specimens, which were identified as B. fragilis, B. thetaiotaomicron, B. ovatus, B. caccae, B. uniformis, B. stercoris, B. vulgatus, B. distasonis, and B. merdae by conventional phenotypic characterization, 62 (95%) were correctly identified with this hybridization procedure.
摘要
开发了一种斑点印迹杂交程序，以32P标记的模式菌株全染色体DNA作为探针，作为鉴定肠道拟杆菌属物种的快速简便方法。通过轻微抽吸将细菌细胞固定在膜滤器上，用0.5N NaOH处理，并用这些探针进行杂交。从19份人类粪便标本中分离出65株拟杆菌菌株，通过传统表型特征鉴定为脆弱拟杆菌、多形拟杆菌、卵形拟杆菌、粪拟杆菌、解脲拟杆菌、粪便拟杆菌、普通拟杆菌、狄氏拟杆菌和屎拟杆菌，其中62株（95%）通过该杂交程序得到正确鉴定。
相似文献
1
Rapid and correct identification of intestinal Bacteroides spp. with chromosomal DNA probes by whole-cell dot blot hybridization.通过全细胞斑点印迹杂交用染色体DNA探针快速准确鉴定肠道拟杆菌属菌种。
Appl Environ Microbiol. 1988 May;54(5):1158-62. doi: 10.1128/aem.54.5.1158-1162.1988.
2
Use of randomly cloned DNA fragments for identification of Bacteroides thetaiotaomicron.使用随机克隆的DNA片段鉴定嗜内脏拟杆菌。
J Bacteriol. 1983 Apr;154(1):287-93. doi: 10.1128/jb.154.1.287-293.1983.
3
Use of a species-specific DNA hybridization probe for enumerating Bacteroides vulgatus in human feces.使用物种特异性DNA杂交探针计数人粪便中的普通拟杆菌。
Appl Environ Microbiol. 1985 Oct;50(4):958-64. doi: 10.1128/aem.50.4.958-964.1985.
4
Enumeration of polysaccharide-degrading Bacteroides species in human feces by using species-specific DNA probes.使用种特异性DNA探针计数人粪便中降解多糖的拟杆菌属物种。
Appl Environ Microbiol. 1986 Feb;51(2):385-90. doi: 10.1128/aem.51.2.385-390.1986.
5
DNA probes for identification of clinically important Bacteroides species.用于鉴定临床重要拟杆菌属菌种的DNA探针。
J Clin Microbiol. 1986 Feb;23(2):343-9. doi: 10.1128/jcm.23.2.343-349.1986.
6
Application of quantitative real-time PCR for rapid identification of Bacteroides fragilis group and related organisms in human wound samples.定量实时 PCR 在人伤口样本中快速鉴定脆弱拟杆菌群及相关生物的应用。
Anaerobe. 2011 Apr;17(2):64-8. doi: 10.1016/j.anaerobe.2011.03.004. Epub 2011 Mar 23.
7
Chromosomal DNA probes for the identification of Bacteroides species.用于鉴定拟杆菌属菌种的染色体DNA探针。
J Gen Microbiol. 1987 Jun;133(6):1423-30. doi: 10.1099/00221287-133-6-1423.
8
Rapid differentiation of the species of the genus Bacteroides sensu stricto by capillary gas chromatography of cellular carbohydrates.通过细胞碳水化合物的毛细管气相色谱法对狭义拟杆菌属的菌种进行快速鉴别。
J Appl Bacteriol. 1991 Mar;70(3):216-20. doi: 10.1111/j.1365-2672.1991.tb02927.x.
9
Identification of feces by detection of Bacteroides genes.通过检测拟杆菌基因鉴定粪便。
Forensic Sci Int Genet. 2013 Jan;7(1):176-9. doi: 10.1016/j.fsigen.2012.09.006. Epub 2012 Oct 12.
10
The isolation and identification of Bacteroides spp. from the normal human faecal flora.从正常人类粪便菌群中分离和鉴定拟杆菌属。
J Med Microbiol. 1980 Feb;13(1):69-78. doi: 10.1099/00222615-13-1-69.
引用本文的文献
1
Reverse sample genome probing, a new technique for identification of bacteria in environmental samples by DNA hybridization, and its application to the identification of sulfate-reducing bacteria in oil field samples.反转样品基因组探测，一种通过 DNA 杂交鉴定环境样品中细菌的新技术，及其在油田样品中硫酸盐还原菌鉴定中的应用。
Appl Environ Microbiol. 1991 Nov;57(11):3070-8. doi: 10.1128/aem.57.11.3070-3078.1991.
2
Effect of polyethylene glycol-electrolyte lavage solution on intestinal microflora.聚乙二醇电解质灌洗液对肠道微生物群的影响。
Appl Environ Microbiol. 1989 Apr;55(4):1026-8. doi: 10.1128/aem.55.4.1026-1028.1989.
3
Oligonucleotide probe for detection and identification of Campylobacter pylori.用于检测和鉴定幽门螺杆菌的寡核苷酸探针
J Clin Microbiol. 1989 Dec;27(12):2652-5. doi: 10.1128/jcm.27.12.2652-2655.1989.
本文引用的文献
1
PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT.通过苯酚处理制备转化脱氧核糖核酸
Biochim Biophys Acta. 1963 Aug 20;72:619-29.
2
An antigenic analysis of Lactobacillus acidophilus.嗜酸乳杆菌的抗原分析。
J Infect Dis. 1962 May-Jun;110:258-67. doi: 10.1093/infdis/110.3.258.
3
Improved medium for selective isolation and enumeration of Bifidobacterium.用于双歧杆菌选择性分离和计数的改良培养基。
Appl Environ Microbiol. 1980 Nov;40(5):866-9. doi: 10.1128/aem.40.5.866-869.1980.
4
Production of a fecal mutagen by Bacteroides spp.拟杆菌属产生粪便诱变剂
Infect Immun. 1982 Sep;37(3):975-80. doi: 10.1128/iai.37.3.975-980.1982.
5
Use of randomly cloned DNA fragments for identification of Bacteroides thetaiotaomicron.使用随机克隆的DNA片段鉴定嗜内脏拟杆菌。
J Bacteriol. 1983 Apr;154(1):287-93. doi: 10.1128/jb.154.1.287-293.1983.
6
The isolation and identification of Bacteroides spp. from the normal human faecal flora.从正常人类粪便菌群中分离和鉴定拟杆菌属。
J Med Microbiol. 1980 Feb;13(1):69-78. doi: 10.1099/00222615-13-1-69.
7
Human fecal flora: the normal flora of 20 Japanese-Hawaiians.人类粪便菌群：20名日裔夏威夷人的正常菌群。
Appl Microbiol. 1974 May;27(5):961-79. doi: 10.1128/am.27.5.961-979.1974.
8
Use of a species-specific DNA hybridization probe for enumerating Bacteroides vulgatus in human feces.使用物种特异性DNA杂交探针计数人粪便中的普通拟杆菌。
Appl Environ Microbiol. 1985 Oct;50(4):958-64. doi: 10.1128/aem.50.4.958-964.1985.
9
Mutagenic activation of biliary metabolites of 1-nitropyrene by intestinal microflora.
Mutat Res. 1985 Apr;149(2):171-8. doi: 10.1016/0027-5107(85)90023-5.
10
Enumeration of polysaccharide-degrading Bacteroides species in human feces by using species-specific DNA probes.使用种特异性DNA探针计数人粪便中降解多糖的拟杆菌属物种。
Appl Environ Microbiol. 1986 Feb;51(2):385-90. doi: 10.1128/aem.51.2.385-390.1986.
Zotero 插件