氯化硝基芳香化合物的酶促脱卤作用。
Enzymatic dehalogenation of chlorinated nitroaromatic compounds.
作者信息
Thiele J, Müller R, Lingens F
机构信息
Institut für Mikrobiologie, Universität Hohenheim, Stuttgart, Federal Republic of Germany.
出版信息
Appl Environ Microbiol. 1988 May;54(5):1199-202. doi: 10.1128/aem.54.5.1199-1202.1988.
Abstract
4-Chlorobenzoate dehalogenase from Pseudomonas sp. strain CBS3 converted 4-chloro-3,5-dinitrobenzoate to 3,5-dinitro-4-hydroxybenzoate and 1-chloro-2,4-dinitrobenzene to 2,4-dinitrophenol. The activities were 0.13 mU/mg of protein for 4-chloro-3,5-dinitrobenzoate and 0.16 mU/mg of protein for 1-chloro-2,4-dinitrobenzene compared with 0.5 mU/mg of protein for 4-chlorobenzoate.
摘要
来自假单胞菌属菌株CBS3的4-氯苯甲酸脱卤酶可将4-氯-3,5-二硝基苯甲酸转化为3,5-二硝基-4-羟基苯甲酸，将1-氯-2,4-二硝基苯转化为2,4-二硝基苯酚。对于4-氯-3,5-二硝基苯甲酸，酶活性为0.13 mU/mg蛋白质；对于1-氯-2,4-二硝基苯，酶活性为0.16 mU/mg蛋白质，而对于4-氯苯甲酸，酶活性为0.5 mU/mg蛋白质。
相似文献
1
Enzymatic dehalogenation of chlorinated nitroaromatic compounds.氯化硝基芳香化合物的酶促脱卤作用。
Appl Environ Microbiol. 1988 May;54(5):1199-202. doi: 10.1128/aem.54.5.1199-1202.1988.
2
Dehalogenation of 4-chlorobenzoate by 4-chlorobenzoate dehalogenase from pseudomonas sp. CBS3: an ATP/coenzyme A dependent reaction.假单胞菌属CBS3的4-氯苯甲酸脱卤酶对4-氯苯甲酸的脱卤作用：一种依赖ATP/辅酶A的反应。
Biochem Biophys Res Commun. 1991 May 15;176(3):1106-11. doi: 10.1016/0006-291x(91)90398-q.
3
Cloning of Pseudomonas sp. strain CBS3 genes specifying dehalogenation of 4-chlorobenzoate.假单胞菌属CBS3菌株中负责4-氯苯甲酸脱卤作用的基因的克隆
J Bacteriol. 1986 Oct;168(1):81-5. doi: 10.1128/jb.168.1.81-85.1986.
4
Dehalogenation of 4-chlorobenzoate. Characterisation of 4-chlorobenzoyl-coenzyme A dehalogenase from Pseudomonas sp. CBS3.4-氯苯甲酸的脱卤作用。来自假单胞菌属CBS3的4-氯苯甲酰辅酶A脱卤酶的特性
Biodegradation. 1995 Sep;6(3):203-12. doi: 10.1007/BF00700458.
5
Metabolism of both 4-chlorobenzoate and toluene under denitrifying conditions by a constructed bacterial strain.一株构建的细菌菌株在反硝化条件下对4-氯苯甲酸和甲苯的代谢
Appl Environ Microbiol. 1994 Mar;60(3):989-95. doi: 10.1128/aem.60.3.989-995.1994.
6
Identification of 4-chlorobenzoyl-coenzyme A as intermediate in the dehalogenation catalyzed by 4-chlorobenzoate dehalogenase from Pseudomonas sp. CBS3.鉴定4-氯苯甲酰辅酶A为假单胞菌CBS3中4-氯苯甲酸脱卤酶催化脱卤反应的中间体。
FEBS Lett. 1991 Sep 23;290(1-2):224-6. doi: 10.1016/0014-5793(91)81265-a.
7
Novel enzymic hydrolytic dehalogenation of a chlorinated aromatic.一种氯化芳烃的新型酶促水解脱卤反应
Science. 1991 Jul 12;253(5016):182-5. doi: 10.1126/science.1853203.
8
Cloning and sequence analysis of genes for dehalogenation of 4-chlorobenzoate from Arthrobacter sp. strain SU.节杆菌属菌株SU中4-氯苯甲酸脱卤基因的克隆与序列分析
Appl Environ Microbiol. 1992 Dec;58(12):4068-71. doi: 10.1128/aem.58.12.4068-4071.1992.
9
On the origins and functions of the enzymes of the 4-chlorobenzoate to 4-hydroxybenzoate converting pathway.关于4-氯苯甲酸向4-羟基苯甲酸转化途径中酶的起源和功能
Biodegradation. 1994 Dec;5(3-4):259-76. doi: 10.1007/BF00696464.
10
Cloning and sequencing of the fcbB gene encoding 4-chlorobenzoate-coenzyme A dehalogenase from Pseudomonas sp. DJ-12.
Mol Cells. 1999 Apr 30;9(2):225-9.
引用本文的文献
1
Novel partial reductive pathway for 4-chloronitrobenzene and nitrobenzene degradation in Comamonas sp. strain CNB-1.丛毛单胞菌属菌株CNB-1中4-氯硝基苯和硝基苯降解的新型部分还原途径。
Appl Environ Microbiol. 2006 Mar;72(3):1759-65. doi: 10.1128/AEM.72.3.1759-1765.2006.
2
Supplemental substrate enhancement of 2,4-dinitrophenol mineralization by a bacterial consortium.细菌协同作用对 2,4-二硝基苯酚矿化的补充基质增强作用。
Appl Environ Microbiol. 1990 Jun;56(6):1551-8. doi: 10.1128/aem.56.6.1551-1558.1990.
3
Evolution of a pathway for chlorobenzene metabolism leads to natural attenuation in contaminated groundwater.氯苯代谢途径的演变导致污染地下水中的自然衰减。
Appl Environ Microbiol. 1998 Nov;64(11):4185-93. doi: 10.1128/AEM.64.11.4185-4193.1998.
4
Degradation of halogenated aromatic compounds.卤代芳香化合物的降解
Biodegradation. 1990;1(2-3):207-20. doi: 10.1007/BF00058837.
本文引用的文献
1
Nitrosubstituted aromatic compounds as nitrogen source for bacteria.亚硝基取代芳香族化合物作为细菌的氮源。
Appl Environ Microbiol. 1987 Jan;53(1):208-10. doi: 10.1128/aem.53.1.208-210.1987.
2
Mechanisms of the reductive denitration of pentachloronitrobenzene (PCNB) and the reductive dechlorination of hexachlorobenzene (HCB).五氯硝基苯（PCNB）的还原脱硝和六氯苯（HCB）的还原脱氯机制。
Xenobiotica. 1984 Sep;14(9):705-10. doi: 10.3109/00498258409151468.
3
Incorporation of [18O]water into 4-hydroxybenzoic acid in the reaction of 4-chlorobenzoate dehalogenase from pseudomonas spec. CBS 3.在假单胞菌属CBS 3的4-氯苯甲酸脱卤酶反应中，[18O]水掺入4-羟基苯甲酸中。
Biochem Biophys Res Commun. 1984 Oct 15;124(1):178-82. doi: 10.1016/0006-291x(84)90933-1.
4
[The microbial degradation of chloramphenicol].[氯霉素的微生物降解]
Biochim Biophys Acta. 1966 Dec 28;130(2):345-54.
5
6
Influence of para-substituents on the oxidative metabolism of o-nitrophenols by Pseudomonas putida B2.对位取代基对恶臭假单胞菌B2氧化代谢邻硝基苯酚的影响。
Appl Environ Microbiol. 1986 Aug;52(2):334-9. doi: 10.1128/aem.52.2.334-339.1986.
7
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.一种利用蛋白质 - 染料结合原理对微克级蛋白质进行定量的快速灵敏方法。
Anal Biochem. 1976 May 7;72:248-54. doi: 10.1016/0003-2697(76)90527-3.
8
Metabolism of nitrophenols by bacteria isolated from parathion-amended flooded soil.
Antonie Van Leeuwenhoek. 1976;42(4):461-70. doi: 10.1007/BF00410177.
9
Microbial transformation of 2,4,6-trinitrotoluene and other nitroaromatic compounds.2,4,6-三硝基甲苯及其他硝基芳香族化合物的微生物转化
Appl Environ Microbiol. 1976 Jun;31(6):949-58. doi: 10.1128/aem.31.6.949-958.1976.
10
Metabolism of 3-chloro-, 4-chloro-, and 3,5-dichlorobenzoate by a pseudomonad.一株假单胞菌对3-氯苯甲酸、4-氯苯甲酸和3,5-二氯苯甲酸的代谢
Appl Environ Microbiol. 1979 Mar;37(3):421-8. doi: 10.1128/aem.37.3.421-428.1979.
Zotero 插件