Zappe H, Jones W A, Jones D T, Woods D R
Department of Microbiology, University of Cape Town, Rondebosch, South Africa.
Appl Environ Microbiol. 1988 May;54(5):1289-92. doi: 10.1128/aem.54.5.1289-1292.1988.
The nucleotide sequence of an endo-beta-1,4-glucanase gene of Clostridium acetobutylicum contained two putative extended promoter consensus sequences, a Shine-Dalgarno sequence and a TTG initiation codon. The nucleotide sequence of the gene coding for the C-terminal region of this enzyme was not required for activity. Extensive homology in the nucleotide and amino acid sequences of the endoglucanase genes from C. acetobutylicum and Bacillus spp. was demonstrated.
丙酮丁醇梭菌的一种内切-β-1,4-葡聚糖酶基因的核苷酸序列包含两个推定的扩展启动子共有序列、一个SD序列和一个TTG起始密码子。该酶C端区域编码基因的核苷酸序列对活性而言并非必需。已证明丙酮丁醇梭菌和芽孢杆菌属的内切葡聚糖酶基因在核苷酸和氨基酸序列上有广泛的同源性。