• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

嗜碱芽孢杆菌N-4菌株中两个纤维素酶基因的核苷酸序列及其高度同源性。

Nucleotide sequences of two cellulase genes from alkalophilic Bacillus sp. strain N-4 and their strong homology.

作者信息

Fukumori F, Sashihara N, Kudo T, Horikoshi K

出版信息

J Bacteriol. 1986 Nov;168(2):479-85. doi: 10.1128/jb.168.2.479-485.1986.

DOI:10.1128/jb.168.2.479-485.1986
PMID:3782013
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC213507/
Abstract

Two genes for cellulases of alkalophilic Bacillus sp. strain N-4 (ATCC 21833) have been sequenced. From the DNA sequences the cellulases encoded in the plasmids pNK1 and pNK2 consist of 488 and 409 amino acids, respectively. The DNA and protein sequences of the pNK1-encoded cellulase are related to those of the pNK2-encoded cellulase. The pNK2-encoded cellulase lacks the direct repeat sequence of a stretch of 60 amino acids near the C-terminal end of the pNK1-encoded cellulase. The duplication of the cellulase genes and the formation of the direct repeat in the pNK1-encoded cellulase occurred at almost the same time.

摘要

已对嗜碱芽孢杆菌N-4菌株（ATCC 21833）的两种纤维素酶基因进行了测序。根据DNA序列，质粒pNK1和pNK2中编码的纤维素酶分别由488和409个氨基酸组成。pNK1编码的纤维素酶的DNA和蛋白质序列与pNK2编码的纤维素酶的序列相关。pNK2编码的纤维素酶在pNK1编码的纤维素酶C末端附近缺少一段60个氨基酸的直接重复序列。纤维素酶基因的复制以及pNK1编码的纤维素酶中直接重复序列的形成几乎是同时发生的。

相似文献

1
Nucleotide sequences of two cellulase genes from alkalophilic Bacillus sp. strain N-4 and their strong homology.嗜碱芽孢杆菌N-4菌株中两个纤维素酶基因的核苷酸序列及其高度同源性。
J Bacteriol. 1986 Nov;168(2):479-85. doi: 10.1128/jb.168.2.479-485.1986.
2
Molecular cloning and expression of cellulase genes of alkalophilic Bacillus sp. strain N-4 in Escherichia coli.嗜碱芽孢杆菌N-4菌株纤维素酶基因在大肠杆菌中的分子克隆与表达
J Bacteriol. 1984 May;158(2):503-6. doi: 10.1128/jb.158.2.503-506.1984.
3
The third cellulase of alkalophilic Bacillus sp. strain N-4: evolutionary relationships within the cel gene family.嗜碱芽孢杆菌N-4菌株的第三种纤维素酶：cel基因家族内的进化关系
Gene. 1989;76(2):289-98. doi: 10.1016/0378-1119(89)90169-8.
4
Molecular cloning and nucleotide sequence of the alkaline cellulase gene from the alkalophilic Bacillus sp. strain 1139.嗜碱芽孢杆菌菌株1139碱性纤维素酶基因的分子克隆及核苷酸序列
J Gen Microbiol. 1986 Aug;132(8):2329-35. doi: 10.1099/00221287-132-8-2329.
5
Molecular cloning and nucleotide sequence of a gene for alkaline cellulase from Bacillus sp. KSM-635.芽孢杆菌属KSM-635碱性纤维素酶基因的分子克隆及核苷酸序列
J Gen Microbiol. 1990 Jul;136(7):1327-34. doi: 10.1099/00221287-136-7-1327.
6
Homology between endoglucanase Z of Erwinia chrysanthemi and endoglucanases of Bacillus subtilis and alkalophilic Bacillus.菊欧文氏菌内切葡聚糖酶Z与枯草芽孢杆菌及嗜碱芽孢杆菌内切葡聚糖酶之间的同源性
Mol Microbiol. 1988 Jan;2(1):159-64. doi: 10.1111/j.1365-2958.1988.tb00017.x.
7
Nucleotide sequence of the gene for an alkaline endoglucanase from an alkalophilic Bacillus and its expression in Escherichia coli and Bacillus subtilis.嗜碱芽孢杆菌碱性内切葡聚糖酶基因的核苷酸序列及其在大肠杆菌和枯草芽孢杆菌中的表达
Biosci Biotechnol Biochem. 1992 Jun;56(6):872-7. doi: 10.1271/bbb.56.872.
8
DNA sequences of three beta-1,4-endoglucanase genes from Thermomonospora fusca.来自嗜热栖热放线菌的三个β-1,4-内切葡聚糖酶基因的DNA序列。
J Bacteriol. 1991 Jun;173(11):3397-407. doi: 10.1128/jb.173.11.3397-3407.1991.
9
Molecular cloning and nucleotide sequence of the gene encoding an endo-1,4-beta-glucanase from Bacillus sp. KSM-330.芽孢杆菌属KSM - 330内切 - 1,4 - β - 葡聚糖酶编码基因的分子克隆及核苷酸序列
J Gen Microbiol. 1991 Oct;137(10):2299-305. doi: 10.1099/00221287-137-10-2299.
10
[Cloning and DNA sequencing of Bacillus pumilus endo-1,4-beta-glucanase gene].短小芽孢杆菌内切-1,4-β-葡聚糖酶基因的克隆与DNA测序
Wei Sheng Wu Xue Bao. 2001 Feb;41(1):76-81.

引用本文的文献

1
Molecular Characterization of Three B-1,4-Endoglucanase Genes in and Functional Analysis of -2 Gene.三种β-1,4-内切葡聚糖酶基因的分子特征及β-2基因的功能分析
Plants (Basel). 2021 Mar 17;10(3):568. doi: 10.3390/plants10030568.
2
Avocado cellulase: nucleotide sequence of a putative full-length cDNA clone and evidence for a small gene family.鳄梨纤维素酶：全长 cDNA 克隆的核苷酸序列及小基因家族的证据。
Plant Mol Biol. 1987 May;9(3):197-203. doi: 10.1007/BF00166456.
3
Genomic and enzymatic results show Bacillus cellulosilyticus uses a novel set of LPXTA carbohydrases to hydrolyze polysaccharides.基因组和酶学结果表明，解纤维梭菌利用一套新型的 LPXTA 碳水化合物酶来水解多糖。
PLoS One. 2013 Apr 4;8(4):e61131. doi: 10.1371/journal.pone.0061131. Print 2013.
4
Differential Expression of Xylanases and Endoglucanases in the Hybrid Derived from Intergeneric Protoplast Fusion between a Cellulomonas sp. and Bacillus subtilis.木聚糖酶和内切葡聚糖酶在纤维素单胞菌属和枯草芽孢杆菌属原生质体属间融合杂种中的差异表达。
Appl Environ Microbiol. 1989 Oct;55(10):2675-80. doi: 10.1128/aem.55.10.2675-2680.1989.
5
Cloning, sequencing, and expression of the chitinase gene chiA74 from Bacillus thuringiensis.苏云金芽孢杆菌几丁质酶基因chiA74的克隆、测序及表达
Appl Environ Microbiol. 2003 Feb;69(2):1023-9. doi: 10.1128/AEM.69.2.1023-1029.2003.
6
Identification and characterization of the three chitin-binding domains within the multidomain chitinase Chi92 from Aeromonas hydrophila JP101.嗜水气单胞菌JP101多结构域几丁质酶Chi92中三个几丁质结合结构域的鉴定与表征
Appl Environ Microbiol. 2001 Nov;67(11):5100-6. doi: 10.1128/AEM.67.11.5100-5106.2001.
7
Alkaliphiles: some applications of their products for biotechnology.嗜碱菌：其产物在生物技术中的一些应用
Microbiol Mol Biol Rev. 1999 Dec;63(4):735-50, table of contents. doi: 10.1128/MMBR.63.4.735-750.1999.
8
Molecular and biochemical characterization of two xylanase-encoding genes from Cellulomonas pachnodae.来自豆象纤维单胞菌的两个木聚糖酶编码基因的分子和生化特性
Appl Environ Microbiol. 1999 Sep;65(9):4099-107. doi: 10.1128/AEM.65.9.4099-4107.1999.
9
Characterization of chitinase C from a marine bacterium, Alteromonas sp. strain O-7, and its corresponding gene and domain structure.来自海洋细菌嗜 Alteromonas sp. 菌株 O-7 的几丁质酶 C 的特性及其相应的基因和结构域结构。
Appl Environ Microbiol. 1998 Feb;64(2):472-8. doi: 10.1128/AEM.64.2.472-478.1998.
10
Cloning, sequencing, and expression of the gene encoding Clostridium paraputrificum chitinase ChiB and analysis of the functions of novel cadherin-like domains and a chitin-binding domain.副腐败梭菌几丁质酶ChiB编码基因的克隆、测序与表达，以及新型钙黏蛋白样结构域和几丁质结合结构域功能分析
J Bacteriol. 1997 Dec;179(23):7306-14. doi: 10.1128/jb.179.23.7306-7314.1997.

本文引用的文献

1
Unique features in the ribosome binding site sequence of the gram-positive Staphylococcus aureus beta-lactamase gene.革兰氏阳性金黄色葡萄球菌β-内酰胺酶基因核糖体结合位点序列的独特特征。
J Biol Chem. 1981 Nov 10;256(21):11283-91.
2
Nucleotide sequence of the 5' region of the Bacillus licheniformis alpha-amylase gene: comparison with the B. amyloliquefaciens gene.地衣芽孢杆菌α-淀粉酶基因5'区域的核苷酸序列：与解淀粉芽孢杆菌基因的比较。
J Bacteriol. 1984 Apr;158(1):369-72. doi: 10.1128/jb.158.1.369-372.1984.
3
Molecular evolution of mRNA: a method for estimating evolutionary rates of synonymous and amino acid substitutions from homologous nucleotide sequences and its application.信使核糖核酸的分子进化：一种从同源核苷酸序列估计同义替换和氨基酸替换进化速率的方法及其应用
J Mol Evol. 1980 Sep;16(1):23-36. doi: 10.1007/BF01732067.
4
Molecular cloning and expression of cellulase genes of alkalophilic Bacillus sp. strain N-4 in Escherichia coli.嗜碱芽孢杆菌N-4菌株纤维素酶基因在大肠杆菌中的分子克隆与表达
J Bacteriol. 1984 May;158(2):503-6. doi: 10.1128/jb.158.2.503-506.1984.
5
New M13 vectors for cloning.用于克隆的新型M13载体。
Methods Enzymol. 1983;101:20-78. doi: 10.1016/0076-6879(83)01005-8.
6
A system for shotgun DNA sequencing.一种用于鸟枪法DNA测序的系统。
Nucleic Acids Res. 1981 Jan 24;9(2):309-21. doi: 10.1093/nar/9.2.309.
7
Nucleotide sequence of the amylase gene from Bacillus subtilis.枯草芽孢杆菌淀粉酶基因的核苷酸序列。
Nucleic Acids Res. 1983 Jan 25;11(2):237-49. doi: 10.1093/nar/11.2.237.
8
The DNA sequence of the gene and genetic control sites for the excreted B. subtilis enzyme beta-glucanase.枯草芽孢杆菌分泌型β-葡聚糖酶的基因及遗传控制位点的DNA序列。
Nucleic Acids Res. 1984 Jul 11;12(13):5355-67. doi: 10.1093/nar/12.13.5355.
9
Purification and properties of extracellular and cell-bound cellulase components of Pseudomonas fluorescens var. cellulosa.荧光假单胞菌纤维素变种胞外和细胞结合纤维素酶组分的纯化及性质
J Biochem. 1970 Jan;67(1):19-35. doi: 10.1093/oxfordjournals.jbchem.a129231.
10
Improved estimation of secondary structure in ribonucleic acids.核糖核酸二级结构的改进估计。
Nat New Biol. 1973 Nov 14;246(150):40-1. doi: 10.1038/newbio246040a0.