嗜碱芽孢杆菌N-4菌株中两个纤维素酶基因的核苷酸序列及其高度同源性。
Nucleotide sequences of two cellulase genes from alkalophilic Bacillus sp. strain N-4 and their strong homology.
作者信息
Fukumori F, Sashihara N, Kudo T, Horikoshi K
出版信息
J Bacteriol. 1986 Nov;168(2):479-85. doi: 10.1128/jb.168.2.479-485.1986.
Abstract
Two genes for cellulases of alkalophilic Bacillus sp. strain N-4 (ATCC 21833) have been sequenced. From the DNA sequences the cellulases encoded in the plasmids pNK1 and pNK2 consist of 488 and 409 amino acids, respectively. The DNA and protein sequences of the pNK1-encoded cellulase are related to those of the pNK2-encoded cellulase. The pNK2-encoded cellulase lacks the direct repeat sequence of a stretch of 60 amino acids near the C-terminal end of the pNK1-encoded cellulase. The duplication of the cellulase genes and the formation of the direct repeat in the pNK1-encoded cellulase occurred at almost the same time.
摘要
已对嗜碱芽孢杆菌N-4菌株（ATCC 21833）的两种纤维素酶基因进行了测序。根据DNA序列，质粒pNK1和pNK2中编码的纤维素酶分别由488和409个氨基酸组成。pNK1编码的纤维素酶的DNA和蛋白质序列与pNK2编码的纤维素酶的序列相关。pNK2编码的纤维素酶在pNK1编码的纤维素酶C末端附近缺少一段60个氨基酸的直接重复序列。纤维素酶基因的复制以及pNK1编码的纤维素酶中直接重复序列的形成几乎是同时发生的。
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