通过定性遗传转化和营养测定鉴定牛莫拉菌。
Identification of Moraxella bovis by qualitative genetic transformation and nutritional assays.
作者信息
Juni E, Heym G A, Newcomb R D
机构信息
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109-0620.
出版信息
Appl Environ Microbiol. 1988 May;54(5):1304-6. doi: 10.1128/aem.54.5.1304-1306.1988.
Strains of Moraxella bovis were identified definitively through the combined use of a qualitative genetic transformation assay and determination of the ability of the organism under examination to grow in a defined medium (medium MB). Except for weak transformation by DNA from strains of M. lacunata, M. nonliquefaciens, and M. (Branhamella) ovis, DNA samples from all other members of the genus Moraxella failed to transform either of the two M. bovis auxotrophs used in this study.
通过定性遗传转化试验和测定受试生物体在特定培养基（MB培养基）中生长的能力相结合的方法，最终鉴定出牛莫拉菌菌株。除了来自腔隙莫拉菌、非液化莫拉菌和绵羊莫拉菌（布兰汉菌属）菌株的DNA有较弱的转化作用外，来自莫拉菌属所有其他成员的DNA样本均未能转化本研究中使用的两种牛莫拉菌营养缺陷型菌株。
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