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通过定性遗传转化和营养测定鉴定牛莫拉菌。

Identification of Moraxella bovis by qualitative genetic transformation and nutritional assays.

作者信息

Juni E, Heym G A, Newcomb R D

机构信息

Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109-0620.

出版信息

Appl Environ Microbiol. 1988 May;54(5):1304-6. doi: 10.1128/aem.54.5.1304-1306.1988.

DOI:10.1128/aem.54.5.1304-1306.1988
PMID:3389822
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC202648/
Abstract

Strains of Moraxella bovis were identified definitively through the combined use of a qualitative genetic transformation assay and determination of the ability of the organism under examination to grow in a defined medium (medium MB). Except for weak transformation by DNA from strains of M. lacunata, M. nonliquefaciens, and M. (Branhamella) ovis, DNA samples from all other members of the genus Moraxella failed to transform either of the two M. bovis auxotrophs used in this study.

摘要

通过定性遗传转化试验和测定受试生物体在特定培养基(MB培养基)中生长的能力相结合的方法,最终鉴定出牛莫拉菌菌株。除了来自腔隙莫拉菌、非液化莫拉菌和绵羊莫拉菌(布兰汉菌属)菌株的DNA有较弱的转化作用外,来自莫拉菌属所有其他成员的DNA样本均未能转化本研究中使用的两种牛莫拉菌营养缺陷型菌株。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a45/202648/2e3eb0d78d65/aem00110-0243-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a45/202648/42008615e060/aem00110-0243-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a45/202648/2e3eb0d78d65/aem00110-0243-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a45/202648/42008615e060/aem00110-0243-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a45/202648/2e3eb0d78d65/aem00110-0243-b.jpg

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Identification of Moraxella bovis by qualitative genetic transformation and nutritional assays.通过定性遗传转化和营养测定鉴定牛莫拉菌。
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引用本文的文献

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J Clin Microbiol. 1992 Dec;30(12):3099-107. doi: 10.1128/jcm.30.12.3099-3107.1992.

本文引用的文献

1
Infectious bovine keratoconjunctivitis. I. Etiology.牛传染性角膜结膜炎。I. 病因学。
Am J Vet Res. 1960 Sep;21:761-6.
2
A five-year study of infectious bovine keratoconjunctivitis in a beef herd.
J Am Vet Med Assoc. 1970 Aug 15;157(4):443-51.
3
Simple genetic transformation assay for rapid diagnosis of Moraxella osloensis.用于奥斯陆莫拉菌快速诊断的简易基因转化试验
Appl Microbiol. 1974 Jan;27(1):16-24. doi: 10.1128/am.27.1.16-24.1974.
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Interspecies transformation of Acinetobacter: genetic evidence for a ubiquitous genus.不动杆菌的种间转化:一个普遍存在属的遗传学证据
J Bacteriol. 1972 Nov;112(2):917-31. doi: 10.1128/jb.112.2.917-931.1972.
5
Defined medium for Moraxella bovis.牛莫拉菌的特定培养基。
Appl Environ Microbiol. 1986 Oct;52(4):966-8. doi: 10.1128/aem.52.4.966-968.1986.
6
Combined genetic transformation and nutritional assay for identification of Moraxella nonliquefaciens.联合基因转化与营养测定法用于鉴定非液化莫拉菌
J Clin Microbiol. 1987 Sep;25(9):1691-4. doi: 10.1128/jcm.25.9.1691-1694.1987.