Juni E
Appl Microbiol. 1974 Jan;27(1):16-24. doi: 10.1128/am.27.1.16-24.1974.
A genetic transformation assay for unequivocal identification of strains of Moraxella osloensis is described. In this assay a stable tryptophan auxotroph is transformed to prototrophy by deoxyribonucleic acid (DNA) samples from other strains of M. osloensis but not by DNA samples from unrelated bacteria. The test is simple to perform and definitive results can be obtained in less than 24 h. The procedure, which is suitable for routine diagnosis in a clinical laboratory, involves a rapid method for preparation of crude transforming DNA from small quantities of bacterial cells and permits simultaneous examination of large numbers of isolated cultures. The assay was shown to correctly identify 27 strains previously classified as M. osloensis. Forty-five other gram-negative, oxidase-positive, nonmotile coccobacilli, which might be confused with M. osloensis unless subject to more extensive testing, were shown to be unrelated genetically to M. osloensis. The transformation assay clearly distinguishes M. osloensis from Acinetobacter. Although most strains of M. osloensis are nonfastidious, being able to grow in a mineral medium supplemented with a single organic carbon source, one of the strains tested was only able to grow on fairly complex media and could not be transformed to grow on simple media. Inability to alkalize Simmons citrate agar was shown not to be characteristic of all strains of M. osloensis.
本文描述了一种用于明确鉴定奥斯陆莫拉菌菌株的基因转化试验。在该试验中，稳定的色氨酸营养缺陷型菌株可被来自其他奥斯陆莫拉菌菌株的脱氧核糖核酸（DNA）样品转化为原养型，但不能被来自无关细菌的DNA样品转化。该试验操作简单，不到24小时即可获得明确结果。该方法适用于临床实验室的常规诊断，包括一种从少量细菌细胞中快速制备粗转化DNA的方法，并允许同时检测大量分离培养物。该试验被证明能正确鉴定27株先前分类为奥斯陆莫拉菌的菌株。另外45株革兰氏阴性、氧化酶阳性、无动力的球杆菌，除非进行更广泛的检测，否则可能会与奥斯陆莫拉菌混淆，结果显示它们在基因上与奥斯陆莫拉菌无关。该转化试验清楚地将奥斯陆莫拉菌与不动杆菌区分开来。虽然大多数奥斯陆莫拉菌菌株不苛求营养，能够在添加单一有机碳源的矿物培养基中生长，但其中一株受试菌株只能在相当复杂的培养基上生长，无法转化为在简单培养基上生长。结果表明，不能使西蒙斯柠檬酸盐琼脂碱化并非所有奥斯陆莫拉菌菌株的特征。
