Li Xue-Hong, Li Dong-Po, Wu Zhi-Jie, Cui Lei, Xiao Fu-Rong, Li Yong-Hua, Zheng Ye, Zhang Jin-Ming
Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Ying Yong Sheng Tai Xue Bao. 2021 Apr;32(4):1352-1360. doi: 10.13287/j.1001-9332.202104.023.
We studied the effects of urease/nitrification inhibitor combinations on urea hydrolysis and nitrification, aiming to screen out the effective inhibitor combinations for black soil and cinnamon soil in Northeast China. Urease inhibitor, N-butyl thiophosphate-triamine (NBPT), and its combination with nitrification inhibitor dicyandiamide (DCD), 3, 4-dimethylpyrazole phosphate (DMPP), 2-chloro-6 (trichloromethyl)-pyridine (CP), 2-amino-4-chloro-6-methylpyrimidine (AM) and 3-methylpyrazole (MP) were added to urea separately. Samples were collected 15 times in each of all the treatments during 125 days. We examined the changes of urea nitrogen, ammo-nium, nitrate, and nitrification inhibition rate in the two soils. Our results showed the hydrolysis of urea in black soil and cinnamon soil was about 7 d, and the addition of NBPT with or without diffe-rent nitrification inhibitors slowed down the hydrolysis to 21 d at least. Compared with the treatment with common urea, inhibitor addition significantly increased soil NH-N, decreased soil NO-N, and maintained the high NH-N content in soil for a longer time. In black soil, application with nitrification inhibitor inhibited soil nitrification significantly and lasted for more than 125 d. DMPP and CP combined with NBPT increased the NH-N content in black soil by 1.6-1.8 times, while the nitrification inhibition rate was 47.9% and 24.1% at 125 d, respectively. In the cinnamon soil, the application of nitrification inhibitor could prolong the duration of ammonium oxidation from 80 d to 110 d. DCD and DMPP combined with NBPT increased the NH-N content in cinnamon soil by 2.1-3.4 times, while the nitrification inhibition rates at 125 d were 25.3% and 23.2%, respectively. Therefore, NBPT+DMPP combination with urea was recommended for utilization in black soil, followed by NBPT+CP. In cinnamon soil, NBPT+DCD combination with urea was recommended, followed by NBPT+DMPP.
我们研究了脲酶/硝化抑制剂组合对尿素水解和硝化作用的影响，旨在筛选出适合中国东北黑土和褐土的有效抑制剂组合。将脲酶抑制剂N-丁基硫代磷酸三胺（NBPT）及其与硝化抑制剂双氰胺（DCD）、3,4-二甲基吡唑磷酸盐（DMPP）、2-氯-6（三氯甲基）吡啶（CP）、2-氨基-4-氯-6-甲基嘧啶（AM）和3-甲基吡唑（MP）的组合分别添加到尿素中。在125天内，对所有处理的样品进行了15次采集。我们检测了两种土壤中尿素氮、铵、硝酸盐以及硝化抑制率的变化。结果表明，黑土和褐土中尿素的水解时间约为7天，添加NBPT（无论是否与不同硝化抑制剂组合）至少可将水解时间延长至21天。与施用普通尿素的处理相比，添加抑制剂显著增加了土壤铵态氮含量，降低了土壤硝态氮含量，并使土壤中高铵态氮含量维持更长时间。在黑土中，施用硝化抑制剂对土壤硝化作用有显著抑制，且持续时间超过125天。DMPP和CP与NBPT组合使黑土中铵态氮含量提高了1.6 - 1.8倍，在125天时硝化抑制率分别为47.9%和24.1%。在褐土中，施用硝化抑制剂可将铵氧化持续时间从80天延长至110天。DCD和DMPP与NBPT组合使褐土中铵态氮含量提高了2.1 - 3.4倍，在125天时硝化抑制率分别为25.3%和23.2%。因此，建议在黑土中使用NBPT + DMPP与尿素的组合，其次是NBPT + CP。在褐土中，建议使用NBPT + DCD与尿素的组合，其次是NBPT + DMPP。
