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[脲酶/硝化抑制剂对黑土和褐土中尿素氮转化的影响。]

[Effect of urease/nitrification inhibitor on urea nitrogen conversion in black soil and cinnamon soil.].

作者信息

Li Xue-Hong, Li Dong-Po, Wu Zhi-Jie, Cui Lei, Xiao Fu-Rong, Li Yong-Hua, Zheng Ye, Zhang Jin-Ming

机构信息

Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.

University of Chinese Academy of Sciences, Beijing 100049, China.

出版信息

Ying Yong Sheng Tai Xue Bao. 2021 Apr;32(4):1352-1360. doi: 10.13287/j.1001-9332.202104.023.

Abstract

We studied the effects of urease/nitrification inhibitor combinations on urea hydrolysis and nitrification, aiming to screen out the effective inhibitor combinations for black soil and cinnamon soil in Northeast China. Urease inhibitor, N-butyl thiophosphate-triamine (NBPT), and its combination with nitrification inhibitor dicyandiamide (DCD), 3, 4-dimethylpyrazole phosphate (DMPP), 2-chloro-6 (trichloromethyl)-pyridine (CP), 2-amino-4-chloro-6-methylpyrimidine (AM) and 3-methylpyrazole (MP) were added to urea separately. Samples were collected 15 times in each of all the treatments during 125 days. We examined the changes of urea nitrogen, ammo-nium, nitrate, and nitrification inhibition rate in the two soils. Our results showed the hydrolysis of urea in black soil and cinnamon soil was about 7 d, and the addition of NBPT with or without diffe-rent nitrification inhibitors slowed down the hydrolysis to 21 d at least. Compared with the treatment with common urea, inhibitor addition significantly increased soil NH-N, decreased soil NO-N, and maintained the high NH-N content in soil for a longer time. In black soil, application with nitrification inhibitor inhibited soil nitrification significantly and lasted for more than 125 d. DMPP and CP combined with NBPT increased the NH-N content in black soil by 1.6-1.8 times, while the nitrification inhibition rate was 47.9% and 24.1% at 125 d, respectively. In the cinnamon soil, the application of nitrification inhibitor could prolong the duration of ammonium oxidation from 80 d to 110 d. DCD and DMPP combined with NBPT increased the NH-N content in cinnamon soil by 2.1-3.4 times, while the nitrification inhibition rates at 125 d were 25.3% and 23.2%, respectively. Therefore, NBPT+DMPP combination with urea was recommended for utilization in black soil, followed by NBPT+CP. In cinnamon soil, NBPT+DCD combination with urea was recommended, followed by NBPT+DMPP.

摘要

我们研究了脲酶/硝化抑制剂组合对尿素水解和硝化作用的影响,旨在筛选出适合中国东北黑土和褐土的有效抑制剂组合。将脲酶抑制剂N-丁基硫代磷酸三胺(NBPT)及其与硝化抑制剂双氰胺(DCD)、3,4-二甲基吡唑磷酸盐(DMPP)、2-氯-6(三氯甲基)吡啶(CP)、2-氨基-4-氯-6-甲基嘧啶(AM)和3-甲基吡唑(MP)的组合分别添加到尿素中。在125天内,对所有处理的样品进行了15次采集。我们检测了两种土壤中尿素氮、铵、硝酸盐以及硝化抑制率的变化。结果表明,黑土和褐土中尿素的水解时间约为7天,添加NBPT(无论是否与不同硝化抑制剂组合)至少可将水解时间延长至21天。与施用普通尿素的处理相比,添加抑制剂显著增加了土壤铵态氮含量,降低了土壤硝态氮含量,并使土壤中高铵态氮含量维持更长时间。在黑土中,施用硝化抑制剂对土壤硝化作用有显著抑制,且持续时间超过125天。DMPP和CP与NBPT组合使黑土中铵态氮含量提高了1.6 - 1.8倍,在125天时硝化抑制率分别为47.9%和24.1%。在褐土中,施用硝化抑制剂可将铵氧化持续时间从80天延长至110天。DCD和DMPP与NBPT组合使褐土中铵态氮含量提高了2.1 - 3.4倍,在125天时硝化抑制率分别为25.3%和23.2%。因此,建议在黑土中使用NBPT + DMPP与尿素的组合,其次是NBPT + CP。在褐土中,建议使用NBPT + DCD与尿素的组合,其次是NBPT + DMPP。

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