Department of Endodontics, Beijing Stomatological Hospital, School of Stomatology, Capital Medical University, Beijing, 100050, China.
Affiliated Stomatology Hospital of Guangzhou Medical University, School of Stomatology, Guangzhou Medical University, Guangdong, 510140, China.
J Mater Sci Mater Med. 2021 Apr 26;32(5):52. doi: 10.1007/s10856-021-06523-9.
This study is aimed at comparing and evaluating the biocompatibility and antibacterial activities of mineral trioxide aggregate (MTA) and iRoot BP Plus as novel retro-filling materials. Discs of both materials were prepared and incubated for 72 h to obtain material extracts in medium. Flow cytometry and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay were used to assess the rate of apoptosis and proliferation of human periodontal ligament stem cells (hPDLSCs) when exposed to eluates of both materials. The expression levels of alkaline phosphatase, collagen type I, osteocalcin, Runt-related transcription factor-2, and Osterix were tested for evaluating the osteogenic differentiation of hPDLSCs. The antibacterial activities of both materials were compared by the direct contact test. The hPDLSCs stimulated by MTA or iRoot BP Plus eluates showed significantly higher cell viability than that of the control group with no eluates. No significant differences were observed among the percentages of necrotic and apoptotic cells stimulated by MTA and iRoot BP Plus eluates and the control group. The expression of all osteogenic differentiation markers of hPDLSCs in both experimental groups were significantly higher than those of the control group, while the increment values in MTA group were significantly higher than those of the iRoot BP Plus group. The antibacterial activity against Enterococcus faecalis showed no significant difference between MTA and iRoot BP Plus. Therefore, both materials may be suitable for retro-filling applications.
本研究旨在比较和评价矿物三氧化物聚合体（MTA）和 iRoot BP Plus 作为新型根管倒充填材料的生物相容性和抗菌活性。制备两种材料的圆盘，并在培养基中孵育 72 小时以获得材料浸提液。采用流式细胞术和 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐法评估两种材料浸提液对人牙周膜干细胞（hPDLSCs）凋亡和增殖的影响。通过碱性磷酸酶、I 型胶原、骨钙素、Runt 相关转录因子-2 和 Osterix 的表达水平测试来评估 hPDLSCs 的成骨分化。通过直接接触试验比较两种材料的抗菌活性。与无浸提液的对照组相比，MTA 或 iRoot BP Plus 浸提液刺激的 hPDLSCs 的细胞活力明显更高。MTA 和 iRoot BP Plus 浸提液刺激的坏死和凋亡细胞百分比与对照组相比无显著差异。两种实验组的 hPDLSCs 的所有成骨分化标志物的表达均明显高于对照组，而 MTA 组的增量值明显高于 iRoot BP Plus 组。MTA 和 iRoot BP Plus 对粪肠球菌的抗菌活性无显著差异。因此，这两种材料都可能适用于根管倒充填应用。
