一种新型硅酸钙基根管充填材料的体外细胞毒性。

Ex vivo cytotoxicity of a new calcium silicate-based canal filling material.

机构信息

Key Lab for Oral Biomedical Engineering of Ministry of Education, School of Stomatology, Wuhan University, Wuhan, China.

出版信息

Int Endod J. 2010 Sep;43(9):769-74. doi: 10.1111/j.1365-2591.2010.01733.x. Epub 2010 Jun 8.

DOI:10.1111/j.1365-2591.2010.01733.x

PMID:20546044

Abstract

AIM

To evaluate the biocompatibility of iRoot SP root canal filling material to L929 mouse fibroblasts.

METHODOLOGY

Specimens of iRoot SP, AH Plus and ProRoot mineral trioxide aggregate (MTA) were prepared for direct contact using the Millipore filter diffusion test, as well as extracts in MTT assay. Mouse fibroblasts (L929) were used as toxicity targets. Differences in cytotoxicity between fresh and set specimens and between root canal material extracts were determined by t-test. A P-value <0.05 was considered statistically significant.

RESULTS

In the filter diffusion test, iRoot SP was slightly cytotoxic when tested as a fresh mixture and not cytotoxic in a set condition tested after 24 h. AH Plus was rated moderately cytotoxic when tested as a fresh mixture and slightly cytotoxic in a set (24 h) condition. MTA was noncytotoxic in both a fresh or set state. AH Plus was significantly more toxic than iRoot SP and MTA (P < 0.05). In addition, iRoot SP was significantly more toxic than MTA (P < 0.05). In the MTT assay with set specimens, extracts of iRoot SP and MTA were noncytotoxic, whilst extracts of AH Plus were rated as slightly cytotoxic. AH Plus was significantly more toxic than iRoot SP and MTA (P < 0.05).

CONCLUSION

AH Plus root canal sealer was significantly more toxic to L-929 cells than MTA and iRoot SP. iRoot SP had intermediate toxicity.

摘要

目的

评价 iRoot SP 根管封闭剂对 L929 小鼠成纤维细胞的生物相容性。

方法

采用 Millipore 滤膜扩散试验和 MTT 试验，分别将 iRoot SP、AH Plus 和 ProRoot 矿化三氧化物凝聚体（MTA）制成直接接触标本和浸提液。以小鼠成纤维细胞（L929）为毒性靶细胞。采用 t 检验比较新鲜和凝固标本及根管材料浸提液的细胞毒性差异，P 值＜0.05 为差异有统计学意义。

结果

在滤膜扩散试验中，新鲜 iRoot SP 混合物具有轻微细胞毒性，凝固 24 h 后无细胞毒性；新鲜 AH Plus 混合物具有中度细胞毒性，凝固（24 h）后具有轻微细胞毒性；新鲜和凝固状态下的 MTA 均无细胞毒性。新鲜 AH Plus 混合物比 iRoot SP 和 MTA 的细胞毒性更大（P＜0.05），新鲜 iRoot SP 比 MTA 的细胞毒性更大（P＜0.05）。凝固标本 MTT 试验中，iRoot SP 和 MTA 的浸提液无细胞毒性，而 AH Plus 的浸提液具有轻微细胞毒性。凝固 AH Plus 混合物比 iRoot SP 和 MTA 的细胞毒性更大（P＜0.05）。