Płaza Grażyna, Jałowiecki Łukasz, Głowacka Dominika, Hubeny Jakub, Harnisz Monika, Korzeniewska Ewa
Environmental Microbiology Unit, Institute for Ecology of Industrial Areas, Katowice, Poland.
Genomed SA, Warsaw, Poland.
PLoS One. 2021 Apr 26;16(4):e0250514. doi: 10.1371/journal.pone.0250514. eCollection 2021.
Due to limited description of the role and diversity of archaea in WWTPs, the aim of the study was to analyze microbial community structures and diversities with particular regard to Archaea in the samples taken from different stages of the full-scale municipal wastewater treatment plant and effluent receiving water (upstream and downstream discharge point). Our study was focused on showing how the treatment processes influenced the Eubacteria and Archaea composition. Alpha and Beta diversity were used to evaluate the microbial diversity changes in the collected samples. Proteobacteria was the largest fraction ranging from 28% to 67% with 56% relative abundance across all samples. Archaea were present in all stages of WWTP ranged from 1 to 8%. Among the Archaea, two groups of methanogens, acetoclastic (Methanosarcina, Methanosaeta) and hydrogenotrophic methanogens (Methanospirillium, Methanoculleus, Methanobrevibacter) were dominant in the technological stages. The obtained results indicate that the treated wastewater did not significantly affect eubacterial and archaeal composition in receiving water. However, differences in richness, diversity and microbial composition of Eubacteria and Archaea between the wastewater samples taken from the primary and secondary treatment were observed.
由于对污水处理厂中古菌的作用和多样性描述有限，本研究的目的是分析从全尺寸城市污水处理厂不同阶段以及受纳水体（上游和下游排放点）采集的样本中的微生物群落结构和多样性，特别关注古菌。我们的研究重点是展示处理过程如何影响真细菌和古菌的组成。利用α多样性和β多样性来评估所采集样本中微生物多样性的变化。变形菌门是最大的组分，在所有样本中的相对丰度范围为28%至67%，总体相对丰度为56%。古菌存在于污水处理厂的所有阶段，占比为1%至8%。在古菌中，两组产甲烷菌，即乙酸营养型产甲烷菌（甲烷八叠球菌属、甲烷丝状菌属）和氢营养型产甲烷菌（甲烷螺菌属、甲烷袋状菌属、甲烷短杆菌属）在工艺阶段占主导地位。所得结果表明，处理后的废水对受纳水体中的真细菌和古菌组成没有显著影响。然而，观察到从一级处理和二级处理采集的废水样本在真细菌和古菌的丰富度、多样性和微生物组成上存在差异。
