Heart Center & Beijing Key Laboratory of Hypertension, Beijing Chaoyang Hospital, Capital Medical University, 8th Gongtinanlu Rd, Chaoyang District, Beijing, 100020, China.
BMC Microbiol. 2021 Apr 26;21(1):128. doi: 10.1186/s12866-021-02195-1.
Hypertension (HTN) is one of the major cardiovascular risk factors, which contributes to increasing target organ damages and cardiovascular morbidity and mortality worldwide. Isolated systolic HTN (ISH) and isolated diastolic HTN (IDH) are two important subtypes of HTN. Previous researches have demonstrated the alteration of fecal bacteria in HTN, but not down to these two sub-types. In order to identify whether the composition of bacterial taxa and functional modules shift in ISH and IDH, we performed a metagenomic sequencing analysis of fecal samples from 15 controls, 14 ISH, and 11 IDH.
Compared with control and ISH, IDH patients showed decreased gene number, bacterial richness, and evenness, although the bacterial alterations did not reach statistical significance in the Shannon index. Also, at the genus level, the β-diversity for intestinal flora in IDH was distinguishable from those with ISH. Furthermore, the taxonomic composition of ISH or IDH was different from that of healthy control at genus and species levels. Patients with IDH or ISH were confirmed to be enriched with Rothia mucilaginosa, along with reduced Clostridium sp. ASBs410. Lastly, the altered KEGG modules were significantly decreased in IDH compared with the control group, such as sodium transport system; while for ISH, functions relevant to biotin biosynthesis were decreased.
Overall, our results showed the disordered fecal bacteria profiles in subjects with ISH and especially IDH, emphasizing the significance of early intervention for IDH.
高血压（HTN）是主要心血管风险因素之一，它导致全球靶器官损害和心血管发病率和死亡率增加。单纯收缩期高血压（ISH）和单纯舒张期高血压（IDH）是高血压的两种重要亚型。先前的研究已经证明了高血压患者粪便细菌的改变，但没有深入到这两种亚型。为了确定细菌分类群和功能模块在 ISH 和 IDH 中的组成是否发生变化，我们对 15 名对照、14 名 ISH 和 11 名 IDH 患者的粪便样本进行了宏基因组测序分析。
与对照和 ISH 相比，IDH 患者的基因数量、细菌丰富度和均匀度降低，尽管在 Shannon 指数中细菌变化没有达到统计学意义。此外，在属水平上，IDH 患者肠道菌群的 β 多样性与 ISH 患者的肠道菌群明显不同。此外，ISH 或 IDH 患者的分类组成在属和种水平上与健康对照组不同。IDH 或 ISH 患者被证实富含粘罗氏菌，同时减少梭状芽孢杆菌 ASBs410。最后，与对照组相比，IDH 中改变的 KEGG 模块明显减少，如钠转运系统；而对于 ISH，与生物素生物合成相关的功能减少。
总的来说，我们的结果显示了 ISH 患者特别是 IDH 患者粪便细菌谱紊乱，强调了对 IDH 进行早期干预的重要性。
