一种来自拟康氏木霉ACCC30425的耐碱且耐表面活性剂的脂肪酶，在洗涤剂工业中具有很高的应用潜力。
An alkaline and surfactant-tolerant lipase from Trichoderma lentiforme ACCC30425 with high application potential in the detergent industry.
作者信息
Wang Yuzhou, Ma Rui, Li Shigui, Gong Mingbo, Yao Bin, Bai Yingguo, Gu Jingang
机构信息
Key Laboratory of Microbial Resources of the Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
出版信息
AMB Express. 2018 Jun 5;8(1):95. doi: 10.1186/s13568-018-0618-z.
Alkaline lipases with adaptability to low temperatures and strong surfactant tolerance are favorable for application in the detergent industry. In the present study, a lipase-encoding gene, TllipA, was cloned from Trichoderma lentiforme ACCC30425 and expressed in Pichia pastoris GS115. The purified recombinant TlLipA was found to have optimal activities at 50 °C and pH 9.5 and retain stable over the pH range of 6.0-10.0 and 40 °C and below. When using esters of different lengths as substrates, TlLipA showed preference for the medium length p-nitrophenyl octanoate. In comparison to commercial lipases, TlLipA demonstrated higher tolerance to various surfactants (SDS, Tween 20, and Triton X100) and retained more activities after incubation with Triton X100 for up to 24 h. These favorable characteristics make TlLipA prospective as an additive in the detergent industry.
具有低温适应性和强表面活性剂耐受性的碱性脂肪酶有利于在洗涤剂工业中应用。在本研究中，从拟康氏木霉ACCC30425中克隆了一个脂肪酶编码基因TllipA，并在毕赤酵母GS115中表达。发现纯化的重组TlLipA在50°C和pH 9.5时具有最佳活性，并且在6.0-10.0的pH范围内以及40°C及以下保持稳定。当使用不同长度的酯作为底物时，TlLipA表现出对中等长度的对硝基苯基辛酸酯的偏好。与市售脂肪酶相比，TlLipA对各种表面活性剂（SDS、吐温20和曲拉通X100）表现出更高的耐受性，并且在与曲拉通X100孵育长达24小时后保留了更多活性。这些有利特性使TlLipA有望成为洗涤剂工业中的添加剂。
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