From the Department of Radiology and Research Center of Medical Sciences, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, the People's Republic of China (J.L., L.N.); and State Key Laboratory of Molecular Vaccinology and Molecular Diagnosis and Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiang'n South Road, Xiamen 361005, the People's Republic of China (J.L., Y.X., L.X.).
Radiology. 2021 Jul;300(1):89-97. doi: 10.1148/radiol.2021204134. Epub 2021 Apr 27.
Background Dynamic contrast-enhanced (DCE) photoacoustic (PA) imaging (PAI) is a novel noninvasive imaging modality that uses the differences in optical absorption of oxyhemoglobin and deoxyhemoglobin and may be performed in a dynamic fashion to image the indocyanine green (ICG) pharmacokinetics in the liver. Purpose To determine whether DCE PAI parameters (maximum peak time [] and half-life []) derived from the PA liver function curve correlate with fibrosis determined using histopathologic analysis. Materials and Methods Between June 2020 and October 2020, 28 male mice aged 8 weeks were intraperitoneally injected with carbon tetrachloride solution every 2 days to establish a liver fibrosis model. At the 1st, 4th, and 8th week of modeling, the changes in liver structure were monitored dynamically by using a PA technique. The and of ICG were calculated at different pathologic stages and within a control group. Corresponding liver histopathologic results and blood biochemical data were obtained. Spearman rank correlation was used to evaluate the relationship between the DCE PAI results and histologic scores. Results The PA liver function curve showed that the and varied among groups (mean : control group, 9 seconds ± 1.8 [standard deviation]; 1 week, 51 seconds ± 4.4; 4 weeks, 73 seconds ± 5.3; 8 weeks, 104 seconds ± 6.6; < .001) (mean T: control group, 28 seconds ± 6.5; 1 week, 201 seconds ± 12.4; 4 weeks, 285 seconds ± 11; 8 weeks, 318 seconds ± 30.5; < .001). There was a positive correlation between the dynamic parameters ( and ) and the histopathologic scores; the Spearman ρ ratios for the Sirius red and α-smooth muscle actin (αSMA)-positive areas versus the were 0.93 and 0.94 ( < .001 for both), and the Spearman ρ ratios for the Sirius red and αSMA-positive areas versus were 0.87 and 0.89 ( < .001 for both). Conclusion Dynamic contrast-enhanced photoacoustic imaging demonstrated a higher maximum peak time and half-life in mice with induced fibrosis compared with control mice without fibrosis, and these values correlated with histologic measures of fibrosis. © RSNA, 2021
背景 动态对比增强（DCE）光声（PA）成像是一种新型的非侵入性成像方式，它利用氧合血红蛋白和去氧血红蛋白的光吸收差异，并可进行动态成像以测量肝脏中吲哚菁绿（ICG）的药代动力学。目的 确定从 PA 肝功能曲线得出的 DCE PAI 参数（最大峰值时间 [T]和半衰期 [T1/2]）是否与使用组织病理学分析确定的纤维化相关。材料与方法 2020 年 6 月至 2020 年 10 月，28 只 8 周龄雄性小鼠通过腹腔注射四氯化碳溶液每隔 2 天建立肝纤维化模型。在建模的第 1、4 和 8 周，使用 PA 技术动态监测肝结构的变化。在不同的病理阶段和对照组中计算 ICG 的 T 和 T1/2。获得相应的肝组织病理学结果和血液生化数据。Spearman 秩相关用于评估 DCE PAI 结果与组织学评分之间的关系。结果 PA 肝功能曲线显示，各组之间的 T 和 T1/2 存在差异（平均值：对照组，9 秒±1.8[标准差]；1 周，51 秒±4.4；4 周，73 秒±5.3；8 周，104 秒±6.6；<.001）（平均值 T：对照组，28 秒±6.5；1 周，201 秒±12.4；4 周，285 秒±11；8 周，318 秒±30.5；<.001）。动态参数（T 和 T1/2）与组织病理学评分之间存在正相关；天狼猩红和α-平滑肌肌动蛋白（αSMA）阳性面积与 T 的 Spearman ρ 比值分别为 0.93 和 0.94（均<.001），天狼猩红和αSMA 阳性面积与 T1/2 的 Spearman ρ 比值分别为 0.87 和 0.89（均<.001）。结论 与无纤维化的对照小鼠相比，诱导纤维化的小鼠的 DCE PA 成像显示出更高的最大峰值时间和半衰期，这些值与纤维化的组织学测量相关。 © 2021 RSNA
