Ménard R, Zador M
Department of Chemistry, Université de Montréal, Quebec, Canada.
Biophys Chem. 1988 Apr;29(3):263-70. doi: 10.1016/0301-4622(88)85047-6.
The reaction between (dien)PdCl+ and polycytidylic acid was studied using spectroscopic and stopped-flow methods. In neutral solution, the palladium complex binds at the N3 site of the cytosine base and causes a noncooperative disruption of the ordered helical structure of poly(C). Interaction at the phosphate group of the polynucleotide was also demonstrated by using the dye acridine orange as an indicator. The results of this study show that the mechanism previously proposed for cytidine and CMP can be applied to poly(C), taking into account particular features of the polymer (polyelectrolytic nature, structure, etc.). In particular, electrostatic effects seem to play a major role in the interaction with metal ion complexes like (dien)Pd(II).
使用光谱法和停流法研究了（二乙三胺）氯化钯（Ⅱ）（dien）PdCl⁺与聚胞苷酸之间的反应。在中性溶液中，钯配合物在胞嘧啶碱基的N3位点结合，并导致聚（C）有序螺旋结构的非协同破坏。使用吖啶橙染料作为指示剂也证明了在多核苷酸磷酸基团处的相互作用。这项研究的结果表明，先前提出的胞苷和胞苷一磷酸（CMP）的作用机制可以应用于聚（C），同时考虑到聚合物的特殊特征（聚电解质性质、结构等）。特别是，静电效应似乎在与（二乙三胺）钯（Ⅱ）（dien）Pd（II）等金属离子配合物的相互作用中起主要作用。
