Center for Regenerative Medicine, Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia.
Plastic and Reconstructive Surgery, Princess Alexandra Hospital, Brisbane, Australia.
Tissue Eng Part C Methods. 2021 Jun;27(6):366-377. doi: 10.1089/ten.TEC.2020.0387.
Scaffold-guided breast tissue engineering (SGBTE) has the potential to transform reconstructive breast surgery. Currently, there is a deficiency in clinically relevant animal models suitable for studying novel breast tissue engineering concepts. To date, only a small number of large animal studies have been conducted and characterization of these large animal models is poorly described in the literature. Addressing this gap in the literature, this publication comprehensively describes our original porcine model based on the current published literature and the experience gained from previous animal studies conducted by our research group. In a long-term experiment using our model, we investigated our SGBTE approach by implanting 60 additively manufactured bioresorbable scaffolds under the panniculus carnosus muscle along the flanks of 12 pigs over 12 months. Our model has the flexibility to compare multiple treatment modalities where we successfully investigated scaffolds filled with various treatments of immediate and delayed fat graft and augmentation with platelet rich plasma. No wound complications were observed using our animal model. We were able to grow clinically relevant volumes of soft tissue, which validates our model. Our preclinical large animal model is ideally suited to assess different scaffold or hydrogel-driven soft tissue regeneration strategies. Impact statement The ability to regenerate soft tissue through scaffold-guided tissue engineering concepts can transform breast reconstructive surgery. We describe an original preclinical large animal model to study controlled and reproducible scaffold-guided breast tissue engineering (SGBTE) concepts. This model features the flexibility to investigate multiple treatment conditions per animal, making it an efficient model. We have validated our model with a long-term experiment over 12 months, which exceeds other shorter published studies. Our SGBTE concept provides a more clinically relevant approach in terms of breast reconstruction. Future studies using this model will support the translation of SGBTE into clinical practice.
支架引导的乳房组织工程（SGBTE）有可能改变重建乳房手术。目前，缺乏适合研究新型乳房组织工程概念的临床相关动物模型。迄今为止，仅进行了少数大型动物研究，并且这些大型动物模型的特征在文献中描述得很差。为了解决文献中的这一空白，本出版物根据当前发表的文献和我们研究小组以前进行的动物研究的经验，全面描述了我们基于原始猪模型。在使用我们的模型进行的长期实验中，我们通过在 12 头猪的侧腹的皮肌下植入 60 个增材制造的可生物吸收支架，研究了我们的 SGBTE 方法，为期 12 个月。我们的模型具有灵活性，可以比较多种治疗方式，我们成功地研究了填充各种即时和延迟脂肪移植物和富含血小板的血浆增强治疗的支架。我们的动物模型没有观察到伤口并发症。我们能够生长出具有临床意义的软组织体积，验证了我们的模型。我们的临床前大型动物模型非常适合评估不同的支架或水凝胶驱动的软组织再生策略。 影响声明 通过支架引导组织工程概念再生软组织可以改变乳房重建手术。我们描述了一种原始的临床前大型动物模型，用于研究受控和可重复的支架引导乳房组织工程（SGBTE）概念。该模型具有对每只动物进行多种治疗条件进行研究的灵活性，使其成为一种高效的模型。我们已经通过长达 12 个月的长期实验验证了我们的模型，这超过了其他较短的已发表研究。我们的 SGBTE 概念在乳房重建方面提供了更具临床意义的方法。未来使用该模型的研究将支持 SGBTE 向临床实践的转化。
