Hsu T C, Collie C J, Lusby A F, Johnston D A
Mutat Res. 1977 Nov;45(2):233-47. doi: 10.1016/0027-5107(77)90023-9.
A protocol is described for cytogenetic assays of chemical mutagens using mammalian cells in vitro. The system employs continuous drug treatment (3 concentrations) for up to 8 h and recovery-cell populations after pulse treatments with a high dose. Both direct fixation (for recording spindle anomalies in anaphase) and colcemid-hypotonic fixation (for reading metaphase chromosome aberrations) are used in order to estimate the effects of an agent as a mitotic poison and as a clastogen respectively. Some DNA intercalating dyes (acridine orange, quinacrine mustard, neutral red) were found to be highly clastogenic whereas others (quinacrine dihydrochloride, 33258 Hoechst) are not.
本文描述了一种使用体外培养的哺乳动物细胞进行化学诱变剂细胞遗传学分析的实验方案。该系统采用连续药物处理（3种浓度）长达8小时，并在高剂量脉冲处理后对恢复细胞群体进行分析。分别采用直接固定法（用于记录后期纺锤体异常）和秋水仙酰胺-低渗固定法（用于读取中期染色体畸变），以分别评估一种试剂作为有丝分裂毒物和断裂剂的作用。发现一些DNA嵌入染料（吖啶橙、喹吖因氮芥、中性红）具有高度断裂性，而其他一些染料（二盐酸喹吖因、Hoechst 33258）则不具有。
