Japan Society for the Promotion of Science, Chiyoda-ku, Tokyo, 102-0083, Japan.
Chubu University Academy of Emerging Sciences, Kasugai, Aichi, 487-8501, Japan.
Int J Syst Evol Microbiol. 2021 Apr;71(4). doi: 10.1099/ijsem.0.004787.
Three strains (YZ01, YZ02 and YZ03) of Gram-stain-positive, facultatively anaerobic rods were isolated from the forestomach contents collected from a captive male proboscis monkey () at Yokohama Zoo in Japan. Phylogenetic analysis of the 16S rRNA gene sequences revealed that these strains belonged to the genus . Based on the sequence similarity of the 16S rRNA gene, subsp. JCM 15610 was the closest phylogenetic neighbour to YZ01. Sequence analyses of two partial concatenated housekeeping genes, the RNA polymerase alpha subunit () and phenylalanyl-tRNA synthase alpha subunit () also indicated that the novel strains belonged to the genus . The average nucleotide identity and digital DNA-DNA hybridization (dDDH) between subsp. and YZ01 were 85.9 and 31.4 %, respectively. The phylogenetic tree based on the whole genomic data of strains YZ01, YZ02 and YZ03 suggested that these three strains formed a single monophyletic cluster in the genus , indicating that it belonged to a new species. The DNA G+C content of strain YZ01 was 51.6 mol%. The major fatty acids were C and C ω9. Therefore, based on phylogenetic, phenotypic and physiological evidence, strains YZ01, YZ02 and YZ03 represent a novel species of the genus , for which the name sp. nov. is proposed with the type strain YZ01 (=JCM 33769=DSM 110539).
从日本横滨动物园一只圈养雄性长鼻猴的瘤胃内容物中分离到三株革兰氏阳性、兼性厌氧的杆菌，菌株 YZ01、YZ02 和 YZ03。16S rRNA 基因序列的系统发育分析表明，这些菌株属于 属。根据 16S rRNA 基因序列的相似性，YZ01 与 subsp. JCM 15610 是最接近的系统发育近邻。两个部分连接的看家基因 RNA 聚合酶α亚基（）和苯丙氨酸 tRNA 合成酶α亚基（）的序列分析也表明，新菌株属于 属。 subsp. 和 YZ01 之间的平均核苷酸同一性和数字 DNA-DNA 杂交（dDDH）分别为 85.9%和 31.4%。基于菌株 YZ01、YZ02 和 YZ03 的全基因组数据构建的系统发育树表明，这三株菌在 属中形成了一个单一的单系群，表明它们属于一个新的种。菌株 YZ01 的 DNA G+C 含量为 51.6mol%。主要脂肪酸为 C 和 Cω9。因此，根据系统发育、表型和生理证据，菌株 YZ01、YZ02 和 YZ03 代表了 属的一个新种，建议将其命名为 sp. nov.，其模式菌株为 YZ01（=JCM 33769=DSM 110539）。
