sp. nov. 和 sp. nov.，分离自蜜蜂（）的肠道。

sp. nov. and sp. nov., isolated from the gut of honeybee ().

机构信息

College of Life Sciences, Northeast Agricultural University, Harbin 150030, PR China.

出版信息

Int J Syst Evol Microbiol. 2022 Feb;72(2). doi: 10.1099/ijsem.0.005237.

DOI:10.1099/ijsem.0.005237

Abstract

Four Gram-stain-positive bacterial strains were isolated from the gut of honeybee () in China. These strains were characterized using a polyphasic taxonomic approach. The data demonstrated that three of the four strains represented two novel species of the genus , strains F306-1 and F551-2 were designated as the type strains. Results of 16S rRNA gene sequence analysis indicated that strains F306-1, F447 and F551-2 were phylogenetically related to the type strains of and , having 99.1-99.7 % 16S rRNA gene sequence (about 1400 bp) similarities. The phylogenetic tree based on concatenated , , , , , and sequences (4114 bp) and the phylogenomic tree based on whole genome sequences indicated that strains F306-1 and F447 were most closely related to Biut2N, and strain F551-2 was most closely related to Hma2N. Strains F306-1 and F447 shared 99.9 % average nucleotide identity (ANI), 99.7 % digital DNA-DNA hybridization (dDDH) and 99.9 % average amino acid identity (AAI) values, indicating that they belong to the same species. Strain F306-1 exhibited the highest ANI (94.4 %), dDDH (56.7 %) and AAI (94.7 %) values to Biut2N. Strain F551-2 had the highest ANI (94.0 %), dDDH (54.3 %) and AAI (95.8 %) values with Hma2N. Acid production from amygdalin, maltose, starch, gentiobiose and turanose, activity of esterase (C4) and -glucosidase, growth with 3 % NaCl at 37 °C under strict anaerobic condition (on mMRS agar plates), and growth with 1-6% NaCl at 37 °C under aerobic condition (on mMRS agar plates supplemented with 0.05 % cysteine or with 1 % cysteine and 2 % fructose) could differentiate strains F306-1 and F447 from DSM 26262. Acid production from d-glucose, arbutin and gentiobiose, growth with 3 % NaCl at 37 °C under strict anaerobic condition (on mMRS agar plates), and growth at 45 °C under strict anaerobic condition (on mMRS agar plates) could differentiate strain F551-2 from DSM 26263. Based upon the data obtained in the present study, two novel species, sp. nov. and sp. nov., are proposed and the type strains are F306-1 (=LMG 32144=JCM 34361=CCTCC AB 2020300) and F551-2 (=JCM 34502=CCTCC AB 2021027), respectively.

摘要

从中国的蜜蜂肠道中分离到了 4 株革兰氏阳性细菌菌株。这些菌株通过多相分类学方法进行了表征。数据表明，这 4 株菌株中有 3 株代表了两个新的属种，菌株 F306-1 和 F551-2 被指定为模式菌株。16S rRNA 基因序列分析结果表明，菌株 F306-1、F447 和 F551-2 与和的模式菌株亲缘关系较近，其 16S rRNA 基因序列（约 1400bp）相似度为 99.1-99.7%。基于串联的、、、、和序列（4114bp）的系统发育树和基于全基因组序列的系统基因组树表明，菌株 F306-1 和 F447 与 Biut2N 最为密切相关，而菌株 F551-2 与 Hma2N 最为密切相关。菌株 F306-1 和 F447 具有 99.9%的平均核苷酸同一性（ANI）、99.7%的数字 DNA-DNA 杂交（dDDH）和 99.9%的平均氨基酸同一性（AAI）值，表明它们属于同一物种。菌株 F306-1 与 Biut2N 的 ANI（94.4%）、dDDH（56.7%）和 AAI（94.7%）值最高。菌株 F551-2 与 Hma2N 的 ANI（94.0%）、dDDH（54.3%）和 AAI（95.8%）值最高。菌株 F306-1 和 F447 能够从苦杏仁苷、麦芽糖、淀粉、龙胆二糖和棉子糖产酸，酯酶（C4）和 -葡萄糖苷酶活性，在严格厌氧条件下（在 mMRS 琼脂平板上）于 37°C 下用 3%NaCl 生长，以及在有氧条件下（在 mMRS 琼脂平板上补充 0.05%半胱氨酸或 1%半胱氨酸和 2%果糖）用 1-6%NaCl 生长，可将菌株 F306-1 和 F447 与 DSM 26262 区分开来。菌株 F306-1 和 F447 能够从 D-葡萄糖、熊果苷和龙胆二糖产酸，在严格厌氧条件下（在 mMRS 琼脂平板上）用 3%NaCl 生长，以及在严格厌氧条件下（在 mMRS 琼脂平板上）于 37°C 下生长，可将菌株 F551-2 与 DSM 26263 区分开来。基于本研究获得的数据，提出了两个新的种，即 sp. nov.和 sp. nov.，其模式菌株分别为 F306-1（=LMG 32144=JCM 34361=CCTCC AB 2020300）和 F551-2（=JCM 34502=CCTCC AB 2021027）。