Institute of Nuclear Physics, Polish Academy of Science, 31-342, Krakow, Poland.
Institute of Health Sciences, Medical College, University of Rzeszow, Kopisto 2a, 35-959, Rzeszow, Poland.
Sci Rep. 2021 Apr 27;11(1):9079. doi: 10.1038/s41598-021-88640-7.
Carcinogenesis is a multifaceted process of cancer formation. The transformation of normal cells into cancerous ones may be difficult to determine at a very early stage. Therefore, methods enabling identification of initial changes caused by cancer require novel approaches. Although physical spectroscopic methods such as FT-Raman and Fourier Transform InfraRed (FTIR) are used to detect chemical changes in cancer tissues, their potential has not been investigated with respect to carcinogenesis. The study aimed to evaluate the usefulness of FT-Raman and FTIR spectroscopy as diagnostic methods of endometrial cancer carcinogenesis. The results indicated development of endometrial cancer was accompanied with chemical changes in nucleic acid, amide I and lipids in Raman spectra. FTIR spectra showed that tissues with development of carcinogenesis were characterized by changes in carbohydrates and amides vibrations. Principal component analysis and hierarchical cluster analysis of Raman spectra demonstrated similarity of tissues with cancer cells and lesions considered precursor of cancer (complex atypical hyperplasia), however they differed from the control samples. Pearson correlation test showed correlation between cancer and complex atypical hyperplasia tissues and between non-cancerous tissue samples. The results of the study indicate that Raman spectroscopy is more effective in assessing the development of carcinogenesis in endometrial cancer than FTIR.
癌变是癌症形成的一个多方面的过程。正常细胞向癌变的转化在早期阶段可能很难确定。因此，需要新的方法来识别由癌症引起的初始变化。尽管傅立叶变换拉曼（FT-Raman）和傅里叶变换红外（FTIR）等物理光谱方法用于检测癌症组织中的化学变化，但它们在癌变方面的潜力尚未得到研究。本研究旨在评估 FT-Raman 和 FTIR 光谱作为子宫内膜癌癌变诊断方法的有用性。结果表明，子宫内膜癌的发展伴随着拉曼光谱中核酸、酰胺 I 和脂质的化学变化。FTIR 光谱显示，具有癌变发展的组织的碳水化合物和酰胺振动发生变化。拉曼光谱的主成分分析和层次聚类分析表明，具有癌细胞的组织与被认为是癌症前体（复杂非典型增生）的病变相似，但它们与对照样本不同。皮尔逊相关检验显示癌症和复杂非典型增生组织之间以及非癌组织样本之间存在相关性。研究结果表明，与 FTIR 相比，拉曼光谱在评估子宫内膜癌癌变的发展方面更有效。
