Center for Structural Biology and Bioinformatics, Laboratory for the Structure and Function of Biological Membranes, Campus Plaine CP206/02, Université Libre de Bruxelles, Brussels, Belgium.
National Institute for Biological standards and Control, Blanche Lane, South Mimms, Potters Bar, Hertfordshire, UK.
Methods Mol Biol. 2021;2271:361-374. doi: 10.1007/978-1-0716-1241-5_25.
FTIR spectroscopy has been widely used to characterize biopharmaceuticals for many years, in particular to analyze protein structure. More recently, it was demonstrated to be a useful tool to study and compare protein samples in terms of glycosylation. Based on a spectral region specific to carbohydrate absorption, we present here a detailed protocol to compare the FTIR spectra of glycoproteins in terms of global glycosylation level and in terms of glycan composition. This FTIR information is compared to MS information. Both approaches yield consistent results but it appears FTIR analysis is easier and more rapid to perform comparisons.
傅里叶变换红外（FTIR）光谱学多年来已被广泛用于生物制药的特性分析，尤其适用于蛋白质结构的分析。最近，它被证明是一种用于研究和比较糖基化蛋白质样品的有用工具。基于碳水化合物吸收的特定光谱区域，我们在此介绍了一种详细的方案，以根据总体糖基化水平和聚糖组成比较糖蛋白的 FTIR 光谱。该 FTIR 信息与 MS 信息进行比较。这两种方法得出的结果一致，但 FTIR 分析似乎更容易且更快速进行比较。
