Malhotra V, Orci L, Glick B S, Block M R, Rothman J E
Department of Biochemistry, Stanford University, California 94305.
Cell. 1988 Jul 15;54(2):221-7. doi: 10.1016/0092-8674(88)90554-5.
An N-ethylmaleimide-sensitive transport component (NSF) has been purified on the basis of its ability to support transport between Golgi cisternae. We now report that NSF is needed for membrane fusion. Thus, when NSF is withheld from incubations of Golgi stacks with cytosol and ATP, uncoated transport vesicles accumulate. Biochemical experiments confirm this conclusion and reveal that NSF is needed to form the first of two previously described prefusion complexes. NSF, therefore, acts within a cascade in which a vesicle-cisterna complex is matured until it is competent for fusion. We suggest that this reflects the stepwise assembly of a multisubunit "fusion machine" following vesicle attachment.
一种对N - 乙基马来酰亚胺敏感的转运成分（NSF）已基于其支持高尔基体潴泡间转运的能力得到纯化。我们现在报告，膜融合需要NSF。因此，当在高尔基体堆叠与胞质溶胶和ATP的孵育中不加入NSF时，无包被的转运囊泡会积累。生化实验证实了这一结论，并揭示NSF是形成先前描述的两个预融合复合物中的第一个所必需的。因此，NSF在一个级联反应中起作用，在该级联反应中，囊泡 - 潴泡复合物成熟直至具备融合能力。我们认为，这反映了囊泡附着后多亚基“融合机器”的逐步组装。
