总胆固醇和高密度脂蛋白胆固醇的酶法分析：与脂质研究临床项目使用的标准化利伯曼-布赫ard方法的比较。

Enzymatic analysis of total- and HDL-cholesterol: comparison with the standardized Liebermann-Burchard method used by the Lipid Research Clinics program.

作者信息

Bachorik P S, Virgil D G, Derby C, Widman D, McMahon R, Fulwood R P, Ezzati T

机构信息

Department of Pediatrics, Johns Hopkins University School of Hygiene, Baltimore, MD 21205.

出版信息

Clin Chim Acta. 1988 Jun 15;174(3):307-14. doi: 10.1016/0009-8981(88)90057-5.

DOI:10.1016/0009-8981(88)90057-5

PMID:3390958

Abstract

We compared two enzymatic cholesterol methods with the standardized chemical method used in the Lipid Research Clinic's (LRC) program. The methods were used to measure total cholesterol and high density lipoprotein (HDL) cholesterol in heparin-MnCl2 supernatants of 1,812 sera collected over a 16-mth period from subjects who were sampled as part of the Hispanic Health and Nutrition Examination Survey. Thirty percent of the subjects had fasted for 12 h or more before venepuncture. The enzymatic total cholesterol values were 1.4-1.8% lower than the LRC method and both enzymatic methods correlated highly with the LRC method (r greater than 0.97). The enzymatic HDL cholesterol values were 2.4 and 6.4% higher than the LRC method, and the correlation between the enzymatic and LRC methods was greater than 0.93. The differences between the enzymatic and LRC methods were the same in samples from fasting and non-fasting subjects.

摘要

我们将两种酶法胆固醇检测方法与脂质研究诊所（LRC）项目中使用的标准化化学方法进行了比较。这些方法用于测量在16个月期间从作为西班牙裔健康与营养检查调查一部分进行采样的受试者中收集的1812份血清的肝素 - 氯化锰上清液中的总胆固醇和高密度脂蛋白（HDL）胆固醇。30%的受试者在静脉穿刺前禁食12小时或更长时间。酶法总胆固醇值比LRC方法低1.4 - 1.8%，且两种酶法与LRC方法的相关性都很高（r大于0.97）。酶法HDL胆固醇值比LRC方法高2.4%和6.4%，酶法与LRC方法之间的相关性大于0.93。酶法与LRC方法之间的差异在禁食和非禁食受试者的样本中是相同的。