Niedmann P D, Luthe H, Wieland H, Schaper G, Seidel D
Klin Wochenschr. 1983 Feb 1;61(3):133-8. doi: 10.1007/BF01486367.
The widespread use of different methods for the determination of HDL-cholesterol (in Europe: sodium phosphotungstic acid/MgCl2) in connection with enzymatic procedures (in the USA: heparin/MnCl2 followed by the Liebermann-Burchard method) but common reference values makes it necessary to evaluate not only accuracy, specificity, and precision of the precipitation step but also of the subsequent cholesterol determination. A high ratio of serum vs. concentrated precipitation reagent (10:1 V/V) leads to the formation of variable amounts of delta-3.5-cholestadiene. This substance is not recognized by cholesterol oxidase but leads to an 1.6 times overestimation by the Liebermann-Burchard method. Therefore, errors in HDL-cholesterol determination should be considered and differences up to 30% may occur between HDL-cholesterol values determined by the different techniques (heparin/MnCl2 - Liebermann-Burchard and NaPW/MgCl2-CHOD-PAP).
在欧洲，使用不同方法（磷酸钨酸钠/MgCl2）结合酶法程序测定高密度脂蛋白胆固醇；在美国，则是采用肝素/MnCl2然后用利伯曼-布赫ard方法。尽管参考值相同，但由于方法不同，不仅需要评估沉淀步骤的准确性、特异性和精密度，还需评估后续胆固醇测定的相关指标。血清与浓缩沉淀试剂的比例过高（10:1 V/V）会导致生成不同量的δ-3,5-胆甾二烯。这种物质不能被胆固醇氧化酶识别，但会使利伯曼-布赫ard方法的测定结果高估1.6倍。因此，应考虑高密度脂蛋白胆固醇测定中的误差，不同技术（肝素/MnCl2 - 利伯曼-布赫ard法和NaPW/MgCl2-CHOD-PAP法）测定的高密度脂蛋白胆固醇值之间可能会出现高达30%的差异。
