Gupta Richa, Wadhwani K K, Tikku A P, Chandra Anil
Department of Conservative Dentistry and Endodontics, Army Dental Center, Research and Referral Army Hospital, New Delhi, India.
Department of Conservative Dentistry and Endodontics, King George's Medical University, Lucknow, Uttar Pradesh, India.
J Conserv Dent. 2020 Sep-Oct;23(5):451-456. doi: 10.4103/JCD.JCD_466_20. Epub 2021 Feb 10.
The present study was carried out with the objective of evaluation of efficacy of two root canal irrigants (LARGAL ULTRA and Biopure mixture of tetracycline, acid, and detergent [MTAD]) in smear layer removal and sealer penetration along the canal walls and to apprise any change in their efficacy when they were activated with two different types of lasers (neodymium-doped yttrium aluminum garnet [Nd:YAG] and erbium-doped yttrium aluminum garnet [Er:YAG]). The analysis was done with the help of confocal laser scanning microscope.
The curved root canals (>20°) from 140 extracted human molars (negative control, = 20) were prepared to size #25.06 with NaOCl irrigation. Teeth were divided into two experimental groups with sample size 60 each (Group I, II). Groups further divided into subgroups IA, IB, and IC and IIA, IIB, and IIC with sample size twenty each. Group IA, Largal Ultra irrigation without LAI; Group IB, Largal Ultra irrigation with Nd:YAG; Group IC, Largal ultra with Er:YAG; Group IIA, MTAD irrigation without LAI; Group IIB, MTAD irrigation with Nd:YAG; Group IIC, MTAD irrigation with Er:YAG. Teeth were obturated with gutta-percha and fluorescent dye labeled sealer. Transverse sections at 3 mm from root apex were examined with confocal laser scanning microscopy, and the percentage of sealer penetration into dentinal tubules was measured.
Analysis of variance calculated (e.g., one-way between-subjects ANOVA) and Tukey's test was performed for each mean comparison.
Difference between control and Group IIc (65.48 ± 0.57) was highest, followed by control and Group Ic (54.92 ± 0.57) and minimum between Group Ib and Group IIa (0.23 ± 0.57). Order of percent diffusion in the present study was: Group IIc > Group Ic > Group IIb > Group Ib ≈ Group IIa > Group Ia > Control ( <.001).
It was inferred that, among the two irrigating solutions tested, Biopure MTAD is more proficient than Largal Ultra. Laser activation of final irrigating solution has been shown to allow better sealer penetration, with Er:YAG laser being more potent than Nd:YAG laser.
本研究旨在评估两种根管冲洗剂（LARGAL ULTRA和四环素、酸及去污剂的生物纯混合物[MTAD]）在去除玷污层以及封闭剂沿根管壁渗透方面的效果，并了解当它们用两种不同类型的激光（掺钕钇铝石榴石[Nd:YAG]和掺铒钇铝石榴石[Er:YAG]）激活时其效果的任何变化。借助共聚焦激光扫描显微镜进行分析。
从140颗拔除的人类磨牙（阴性对照，n = 20）中选取弯曲根管（>20°），用次氯酸钠冲洗并预备至25.06号。牙齿分为两个实验组，每组样本量为60（I组、II组）。每组再进一步分为IA、IB和IC亚组以及IIA, IIB和IIC亚组，每组样本量为20。IA组，用Largal Ultra冲洗但不进行激光辅助冲洗（LAI）；IB组，用Largal Ultra冲洗并结合Nd:YAG激光；IC组，用Largal Ultra冲洗并结合Er:YAG激光；IIA组，用MTAD冲洗但不进行LAI；IIB组，用MTAD冲洗并结合Nd:YAG激光；IIC组，用MTAD冲洗并结合Er:YAG激光。用牙胶和荧光染料标记的封闭剂进行根管充填。在距根尖3mm处制作横向切片，用共聚焦激光扫描显微镜检查，并测量封闭剂渗入牙本质小管的百分比。
计算方差分析（例如，单因素组间方差分析），并对每个均值比较进行Tukey检验。
对照组与IIC组（65.48 ± 0.57）之间的差异最大，其次是对照组与IC组（54.92 ± 0.57），而Ib组与IIa组之间的差异最小（0.23 ± 0.57）。本研究中扩散百分比的顺序为：IIC组>IC组>IIB组>Ib组≈IIa组>IA组>对照组（P <.001）。
可以推断，在所测试的两种冲洗液中，生物纯MTAD比Largal Ultra更有效。已表明最终冲洗液的激光激活能使封闭剂更好地渗透，且Er:YAG激光比Nd:YAG激光更有效。
