Abdelmeguid Nabila E, Khalil Mahmoud I, Badr Nada S, Alkhuriji Afrah F, El-Gerbed Mohamed S A, Sultan Ahmed S
Zoology Department, Faculty of Science, Alexandria University, Alexandria, Egypt.
Department of Biological Sciences, Faculty of Science, Beirut Arab University, Lebanon.
Saudi J Biol Sci. 2021 Apr;28(4):2254-2266. doi: 10.1016/j.sjbs.2021.01.016. Epub 2021 Jan 23.
Colostrum, the sole diet for newborns, is an emerging nutraceutical. To date, the chemopreventive effect of Bovine Colostrum against liver injury induced by the potent carcinogen, 7,12-dimethyl-Benz[a]anthracene (DMBA) is unexplored. Humans are daily exposed to DMBA which is a highly lipophilic environmental organic pollutant. The study aimed to investigate the hepatoprotective role of Bovine Colostrum against DMBA-induced hepatotoxicity using a rat model. Fifty male rats were divided into five groups; GI (control), GII (olive oil, vehicle for DMBA), GIII (DMBA), GIV (DMBA + Bovine Colostrum), GV (Bovine Colostrum). After 12 weeks, body weight changes and mortality were calculated. Histological and ultrastructural examinations of liver tissue were performed. Expressions of p53, TGFβ2, TNF-α, S6K2, and c20orf20 were assessed by RT-PCR. Post-treatment with Bovine Colostrum increased both the body weight and the survival rate of rats treated with DMBA. In addition, remarkable protection against the pathological effect of DMBA was noted. Ultrastructurally, Bovine Colostrum ameliorated/prevented most of the toxic effects of DMBA on hepatocytes, including irregularities of nuclear envelope, clumping, and margination of heterochromatin aggregates, segregated nucleoli, and mitochondrial pleomorphism. Bovine Colostrum administration down-regulated p53, C20orf20, and S6K2 mRNA levels, and up-regulated TNF-α and TGFβ2. In conclusion, Bovine Colostrum have a protective effect against DMBA-induced toxicity on the liver of albino rats. Consequently, Bovine Colostrum may prevent polycyclic aromatic hydrocarbons-induced hepatotoxicity and may be useful in promoting human health if supplemented in the diet.
初乳是新生儿的唯一食物，是一种新兴的营养保健品。迄今为止，牛初乳对由强致癌物7,12 - 二甲基苯并[a]蒽（DMBA）诱导的肝损伤的化学预防作用尚未得到探索。人类每天都会接触到DMBA，它是一种高度亲脂性的环境有机污染物。本研究旨在使用大鼠模型研究牛初乳对DMBA诱导的肝毒性的肝保护作用。将50只雄性大鼠分为五组；GI（对照组），GII（橄榄油，DMBA的载体），GIII（DMBA），GIV（DMBA + 牛初乳），GV（牛初乳）。12周后，计算体重变化和死亡率。对肝组织进行组织学和超微结构检查。通过RT-PCR评估p53、TGFβ2、TNF-α、S6K2和c20orf20的表达。用牛初乳进行治疗后，增加了用DMBA处理的大鼠的体重和存活率。此外，还观察到对DMBA病理效应的显著保护作用。在超微结构上，牛初乳改善/预防了DMBA对肝细胞的大多数毒性作用，包括核膜不规则、异染色质聚集体的聚集和边缘化、核仁分离以及线粒体多形性。给予牛初乳可下调p53、C20orf20和S6K2的mRNA水平，并上调TNF-α和TGFβ2。总之，牛初乳对DMBA诱导的白化大鼠肝脏毒性具有保护作用。因此，牛初乳可能预防多环芳烃诱导的肝毒性，并且如果在饮食中补充可能有助于促进人类健康。
