Alam Pravej
Department of Biology, College of Science and Humanities, Prince Sattam bin Abdulaziz University (PSAU), 11942 Al-Kharj, Saudi Arabia.
Saudi J Biol Sci. 2021 Apr;28(4):2602-2612. doi: 10.1016/j.sjbs.2021.02.005. Epub 2021 Feb 13.
(Bertoni) is known as a natural sweetener plant to produced steviol glycosides. The steviol glycosides biosynthesis is limited in plants due to the alteration in the environmental circumstances such as drought, cold, salt and light. These environmental circumstances are a common side-effect in plants affecting the plant growth, metabolism and yield of secondary metabolites. Due to absence of complete genome annotations, the plant metabolites signaling is difficult in order to get the exact enzymatic flow to the product. In this article, we have analyzed the ESTs of and predicted their role in plant signaling in term of -regulatory elements, their biological function respect to abiotic stress. Further, the predicted abiotic stress responsive factors were also analyzed in order to predict the relevant genes or proteins function in comparison with the genome of . Total 5,548 ESTs of stevia were retrieved from NCBI database. EST-contigs assembled from 5393 were 619 contigs and 2,894 singletons elements were identified by assembler program. Due to short expressed sequences related to singletons, it is excluded for further study. Further, retrieved ESTs were resulted in to 619 EST-contigs by using the clustering method. Out of 619, 15 contigs belongs to transcription factor families while 292 contigs, belongs to five enzyme classes. Out of 619 contigs, the 529 contigs showing the correct gene ontology in term of biological process (BP), molecular Functions (MF) and cellular component (CC). Further, these contigs were also screened for metabolic pathways analyses using KEG database. In this, 390 metabolic pathways and 67 involved for signal transduction were identified from 619 contigs. The co-expression analysis was revealed by gene investigators and STRING 10.0 with 0.40 correlations and 0.9 mutual connection. In projected PPI network, the recognized factors (WD40-like protein, MYB-HB like, AP2-EREBP, C2H2, Hap3/NF-YB, bHLH, C2C2-CO-like, CW-Zn, FHA-SMAD, Nin like, SBP3, TIFY, Tc-PD, Znf-B and bIP) belong to plant signaling and MAPK signaling pathways. These TFs introduce as a candidate genes responsive factors may lead to enhanced plant growth and metabolism by overexpression.
（贝托尼）被认为是一种能产生甜菊糖苷的天然甜味剂植物。由于干旱、寒冷、盐分和光照等环境条件的变化，甜菊糖苷的生物合成在植物中受到限制。这些环境条件是影响植物生长、代谢和次生代谢产物产量的常见副作用。由于缺乏完整的基因组注释，植物代谢物信号传导很难获得精确的酶促流程以生成产物。在本文中，我们分析了（甜菊）的ESTs，并根据调控元件预测了它们在植物信号传导中的作用，以及它们在非生物胁迫方面的生物学功能。此外，还分析了预测的非生物胁迫响应因子，以便与（甜菊）的基因组比较来预测相关基因或蛋白质的功能。从NCBI数据库中检索到甜叶菊总共5548个ESTs。由5393个ESTs组装而成的EST重叠群有619个重叠群，通过组装程序鉴定出2894个单拷贝元件。由于与单拷贝元件相关的表达序列较短，因此将其排除在进一步研究之外。此外，通过聚类方法将检索到的ESTs组装成619个EST重叠群。在619个重叠群中，15个重叠群属于转录因子家族，292个重叠群属于五类酶。在619个重叠群中，529个重叠群在生物学过程（BP）、分子功能（MF）和细胞成分（CC）方面显示出正确的基因本体。此外，还使用KEG数据库对这些重叠群进行代谢途径分析筛选。在此过程中，从619个重叠群中鉴定出390条代谢途径和67条参与信号转导的途径。通过基因研究人员和STRING 10.0进行共表达分析，相关性为0.40，相互连接性为0.9。在预测的蛋白质-蛋白质相互作用网络中，识别出的因子（WD40样蛋白、MYB-HB样、AP2-EREBP、C2H2、Hap3/NF-YB、bHLH、C2C2-CO样、CW-Zn、FHA-SMAD、Nin样、SBP3、TIFY、Tc-PD、Znf-B和bIP）属于植物信号传导和丝裂原活化蛋白激酶信号传导途径。这些作为候选基因响应因子的转录因子可能通过过表达导致植物生长和代谢增强。
