Gerami Mahyar, Majidian Parastoo, Ghorbanpour Akram, Alipour Zeinab
Department of Biology, Sana Institute of Higher Education, Sari, Iran.
Crop and Horticultural Science Research Department, Mazandaran Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Sari, Iran.
Physiol Mol Biol Plants. 2020 May;26(5):965-974. doi: 10.1007/s12298-020-00788-0. Epub 2020 Apr 16.
This study examined the effect of chitosan elicitor with four different concentrations (0, 0.2, 0.4 and 0.6 g/l) on physiological and biochemical properties of stevia under four levels of salinity stress (0, 50, 100, 150 mM level of NaCl). Salt stress caused reduction of chlorophyll (Chl ), chlorophyll (Chl ), total chlorophyll, carotenoid and total protein content. The increment of malondialdehyde (MDA) content was not significant in all NaCl levels, while the CAT and POX activities were increased as well as stevioside and rebaudioside A under salinity stress. On one side, chitosan treatments could compensate the reduction of physiological traits such as photosynthetic pigments and protein content. On the other side, chitosan caused multiple increases in malondialdehyde content, antioxidant enzymes activity (catalase and peroxidase), steviol glycosides (stevioside and rebaudioside A) under salt stress. We report for the first time, the potential of chitosan to enhance salinity-tolerant abilities in stevia through increment of the salt-adaptive factors and to diminish harmful damages caused by NaCl stress.
本研究考察了四种不同浓度（0、0.2、0.4和0.6克/升）的壳聚糖激发子对四个盐胁迫水平（0、50、100、150毫摩尔/升氯化钠水平）下甜叶菊生理生化特性的影响。盐胁迫导致叶绿素a（Chl a）、叶绿素b（Chl b）、总叶绿素、类胡萝卜素和总蛋白含量降低。在所有氯化钠水平下，丙二醛（MDA）含量的增加均不显著，而在盐胁迫下，过氧化氢酶（CAT）和过氧化物酶（POX）活性以及甜菊糖苷和莱鲍迪苷A均有所增加。一方面，壳聚糖处理可以弥补光合色素和蛋白质含量等生理特性的降低。另一方面，壳聚糖在盐胁迫下导致丙二醛含量、抗氧化酶活性（过氧化氢酶和过氧化物酶）、甜菊糖苷（甜菊糖苷和莱鲍迪苷A）多次增加。我们首次报道了壳聚糖通过增加盐适应性因子来增强甜叶菊耐盐能力以及减轻氯化钠胁迫造成的有害损伤的潜力。
