Stukenberg Daniel, Zauner Stefan, Dell'Aquila Gianluca, Maier Uwe G
Department for Cell Biology, Philipps-Universität Marburg, Marburg, Germany.
LOEWE-Zentrum für Synthetische Mikrobiologie (SYNMIKRO), Philipps-Universität Marburg, Marburg, Germany.
Front Plant Sci. 2018 Jun 6;9:740. doi: 10.3389/fpls.2018.00740. eCollection 2018.
CRISPR/Cas9 is a powerful tool for genome editing. We constructed an easy-to-handle expression vector for application in the model organism and tested its capabilities in order to apply CRISPR/Cas9 technology for our purpose. In our experiments, we targeted two different genes, screened for mutations and analyzed mutated diatoms in a three-step process. In the end, we identified cells, showing either monoallelic or homo-biallelic targeted mutations. Thus, we confirm that application of the CRISPR/Cas9 system for is very promising, although, as discussed, overlooked pitfalls have to be considered.
CRISPR/Cas9是一种用于基因组编辑的强大工具。我们构建了一种易于操作的表达载体，用于在模式生物中应用，并测试了其功能，以便将CRISPR/Cas9技术应用于我们的目的。在我们的实验中，我们针对两个不同的基因，通过一个三步过程筛选突变并分析突变的硅藻。最后，我们鉴定出显示单等位基因或双等位基因靶向突变的细胞。因此，我们证实，尽管如所讨论的那样，必须考虑到被忽视的陷阱，但将CRISPR/Cas9系统应用于[此处原文缺失具体应用内容]是非常有前景的。
