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对 P. tricornutum 两种形态的深度比较 RNA 测序揭示了椭圆形形态的特定特征。

Comparative in depth RNA sequencing of P. tricornutum's morphotypes reveals specific features of the oval morphotype.

机构信息

Normandie Univ, UNIROUEN, Laboratoire Glyco-MEV EA4358, 76000, Rouen, France.

Normandie Univ, UNIROUEN, LITIS EA 4108, 76000, Rouen, France.

出版信息

Sci Rep. 2018 Sep 25;8(1):14340. doi: 10.1038/s41598-018-32519-7.

DOI:10.1038/s41598-018-32519-7
PMID:30254372
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6156597/
Abstract

Phaeodactylum tricornutum is the most studied diatom encountered principally in coastal unstable environments. It has been hypothesized that the great adaptability of P. tricornutum is probably due to its pleomorphism. Indeed, P. tricornutum is an atypical diatom since it can display three morphotypes: fusiform, triradiate and oval. Currently, little information is available regarding the physiological significance of this morphogenesis. In this study, we adapted P. tricornutum Pt3 strain to obtain algal culture particularly enriched in one dominant morphotype: fusiform, triradiate or oval. These cultures were used to run high-throughput RNA-Sequencing. The whole mRNA transcriptome of each morphotype was determined. Pairwise comparisons highlighted biological processes and molecular functions which are up- and down-regulated. Finally, intersection analysis allowed us to identify the specific features from the oval morphotype which is of particular interest as it is often described to be more resistant to stresses. This study represent the first transcriptome wide characterization of the three morphotypes from P. tricornutum performed on cultures specifically enriched issued from the same Pt3 strain. This work represents an important step for the understanding of the morphogenesis in P. tricornutum and highlights the particular features of the oval morphotype.

摘要

三角褐指藻是研究最多的一种硅藻，主要存在于沿海不稳定的环境中。人们假设三角褐指藻的高度适应性可能与其多态性有关。事实上，三角褐指藻是一种非典型的硅藻，因为它可以表现出三种形态：梭形、辐射状和椭圆形。目前，关于这种形态发生的生理意义的信息很少。在这项研究中，我们适应三角褐指藻 Pt3 菌株，以获得特别富含一种优势形态的藻类培养物：梭形、辐射状或椭圆形。这些培养物被用于高通量 RNA 测序。每个形态的整个 mRNA 转录组都被确定。成对比较突出了上调和下调的生物学过程和分子功能。最后，交集分析使我们能够识别出椭圆形形态的特定特征，这一点特别有趣，因为它通常被描述为对压力更具抵抗力。本研究首次对 Pt3 菌株来源的特定培养物中三角褐指藻的三种形态进行了全转录组特征描述。这项工作是理解三角褐指藻形态发生的重要一步，突出了椭圆形形态的特殊特征。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0672/6156597/4a56961c5af9/41598_2018_32519_Fig14_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0672/6156597/76d48aa01fb4/41598_2018_32519_Fig6_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0672/6156597/7876adae8220/41598_2018_32519_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0672/6156597/a88d8d8dfae7/41598_2018_32519_Fig11_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0672/6156597/4a56961c5af9/41598_2018_32519_Fig14_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0672/6156597/25064aa599da/41598_2018_32519_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0672/6156597/74942f4f1ba9/41598_2018_32519_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0672/6156597/7d55aaa041b8/41598_2018_32519_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0672/6156597/6c1a4b3d6a40/41598_2018_32519_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0672/6156597/d3b9320a758c/41598_2018_32519_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0672/6156597/76d48aa01fb4/41598_2018_32519_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0672/6156597/752665abcc19/41598_2018_32519_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0672/6156597/04808b33b0ff/41598_2018_32519_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0672/6156597/a59efd5b59c7/41598_2018_32519_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0672/6156597/7876adae8220/41598_2018_32519_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0672/6156597/a88d8d8dfae7/41598_2018_32519_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0672/6156597/fbad304ccc81/41598_2018_32519_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0672/6156597/279aeb4522f0/41598_2018_32519_Fig13_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0672/6156597/4a56961c5af9/41598_2018_32519_Fig14_HTML.jpg

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