Sullivan S G, Stern A, Rosenthal J S, Minkoff L A, Winston A
Department of Psychiatry, Beth Israel Medical Center, New York, NY 10003.
FEBS Lett. 1988 Jul 18;234(2):349-52. doi: 10.1016/0014-5793(88)80114-5.
NMR water-proton spin-lattice relaxation times were studied as probes of water structure in human red blood cells and red blood cell suspensions. Normal saline had a relaxation time of about 3000 ms while packed red blood cells had a relaxation time of about 500 ms. The relaxation time of a red cell suspension at 50% hematocrit was about 750 ms showing that surface charges and polar groups of the red cell membrane effectively structure extracellular water. Incubation of red cells in hypotonic saline increases relaxation time whereas hypertonic saline decreases relaxation time. Relaxation times varied independently of mean corpuscular volume and mean corpuscular hemoglobin concentration in a sample population. Studies with lysates and resealed membrane ghosts show that hemoglobin is very effective in lowering water-proton relaxation time whereas resealed membrane ghosts in the absence of hemoglobin are less effective than intact red cells.
核磁共振水质子自旋晶格弛豫时间作为人类红细胞和红细胞悬液中水结构的探针进行了研究。生理盐水的弛豫时间约为3000毫秒，而浓缩红细胞的弛豫时间约为500毫秒。血细胞比容为50%时红细胞悬液的弛豫时间约为750毫秒，表明红细胞膜的表面电荷和极性基团有效地构成了细胞外水的结构。红细胞在低渗盐水中孵育会增加弛豫时间，而高渗盐水则会降低弛豫时间。在样本群体中，弛豫时间的变化与平均红细胞体积和平均红细胞血红蛋白浓度无关。对裂解物和重新封闭的膜空壳的研究表明，血红蛋白在降低水质子弛豫时间方面非常有效，而在没有血红蛋白的情况下，重新封闭的膜空壳的效果不如完整红细胞。
