Small W C, Goldstein J H
Biochim Biophys Acta. 1981 Jan 22;640(2):430-8. doi: 10.1016/0005-2736(81)90468-5.
A pulse NMR technique employing low extracellular Mn2+ concentrations has been used in following the effect of variations in extracellular osmolality on water transport through the human red blood cell membrane. We report results including the effect of osmolality on the cell water lifetime (tau a) and, for the first time, the effect on the proton spin-spin relaxation of the intracellular water (T2a) and the activation energy for the water transport process. Current results are encouraging in correlating the effects seen in this study with suspected membrane functional changes occurring in both in vivo and in vitro aging and during in vitro preservation attempts.
一种采用低细胞外锰离子浓度的脉冲核磁共振技术，已被用于追踪细胞外渗透压变化对水通过人红细胞膜运输的影响。我们报告的结果包括渗透压对细胞内水寿命（τa）的影响，以及首次报道的对细胞内水质子自旋 - 自旋弛豫（T2a）的影响和水运输过程的活化能。目前的结果对于将本研究中观察到的效应与体内和体外衰老以及体外保存尝试过程中发生的疑似膜功能变化联系起来很有意义。
