Lanzini A, Northfield T C
Department of Medicine, St. George's Hospital Medical School, London, United Kingdom.
Gastroenterology. 1988 Aug;95(2):408-16. doi: 10.1016/0016-5085(88)90498-2.
The aim of this study was to determine the effect of chronic ursodeoxycholic acid administration on coupling of bile acids with cholesterol and phospholipid in hepatic bile, and on cholesterol absorption in the duodenum. A range of bile acid secretion rates was obtained during an evening meal and an overnight fast. Duodenal perfusion of polyethylene glycol as a nonabsorbable recovery marker and of [3H]cholesterol and [14C]lecithin as absorbable recovery markers was combined with continuous intravenous infusion of indocyanine green as a hepatic bile marker. Six subjects with gallstones were studied before and during chronic administration of ursodeoxycholic acid (675 mg/day). Duplicate pretreatment studies revealed good reproducibility for biliary lipid coupling and cholesterol absorption. During ursodeoxycholic acid administration there was a significant alteration not only in bile acid/cholesterol coupling (p less than 0.001), but also in bile acid/phospholipid coupling (p less than 0.001). Mean cholesterol absorption decreased from 25% to 15% (p less than 0.001) during ursodeoxycholic acid administration. These effects of chronic ursodeoxycholic acid administration on biliary lipid coupling are similar to those reported for acute administration, and are thus consistent with an effect caused by bile acid lipid-solubilizing capacity.
本研究的目的是确定长期给予熊去氧胆酸对肝胆汁中胆汁酸与胆固醇及磷脂结合的影响，以及对十二指肠胆固醇吸收的影响。在晚餐期间和夜间禁食期间获得了一系列胆汁酸分泌率。十二指肠灌注聚乙二醇作为不可吸收的回收标记物，灌注[3H]胆固醇和[14C]卵磷脂作为可吸收的回收标记物，并与连续静脉输注吲哚菁绿作为肝胆汁标记物相结合。对6名胆结石患者在长期给予熊去氧胆酸（675毫克/天）之前和期间进行了研究。重复的预处理研究表明，胆汁脂质结合和胆固醇吸收具有良好的可重复性。在给予熊去氧胆酸期间，不仅胆汁酸/胆固醇结合有显著改变（p小于0.001），而且胆汁酸/磷脂结合也有显著改变（p小于0.001）。在给予熊去氧胆酸期间，平均胆固醇吸收从25%降至15%（p小于0.001）。长期给予熊去氧胆酸对胆汁脂质结合的这些影响与急性给药时报道的影响相似，因此与胆汁酸脂质溶解能力引起的效应一致。
