ICAR-Indian Veterinary Research Institute, Eastern Regional Station, Kolkata, India.
Department of Veterinary Microbiology, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, Kolkata, India.
Microb Drug Resist. 2021 Nov;27(11):1457-1469. doi: 10.1089/mdr.2020.0298. Epub 2021 Apr 28.
This study describes comparative occurrence and characterization of multidrug-resistant (MDR) and (KP) in healthy cattle (HC) and cattle with diarrhea (DC) in India. During 2018-2020, 72 MDR isolates, including 35 (DC: 27; HC 8) and 37 (DC: 34; HC: 3), from 251 rectal swabs (DC: 219; HC: 32) were investigated for extended-spectrum beta-lactamase (ESBL), AmpC type β-lactamase and carbapenemase production, antimicrobial susceptibility profile, biofilm production, and efflux pump activity. Fifty-five MDR isolates were ESBL producers (ESBLPs) (DC: 50; HC: 5) and ESBLPs from DC were coresistant to multiple antibiotics. The CTX-M gene (50) was the most frequently detected β-lactamases followed by AmpC (22), TEM1 (13), CMY-6 (6), OXA1 (5), PER (2), DHA, and FOX and SHV12 (1 each). Plasmid-mediated quinolone resistance determinants , , , and were detected in 18, 16, 2, and 3 isolates, respectively. Twenty three isolates revealed mutation in and genes. Tetracycline-resistance markers , , , and were detected in 33, 10, 3, and 2 isolates, respectively. Only one of the 41 imipenem-resistant isolates harbored NDM-5 and two were colistin-resistant. Altogether, 20 MDR isolates were strong biofilm producers and 19 harbored different virulence factors. This is the first ever report from India on the presence of MDR Enterobacteriaceae with resistance to even last-resort antimicrobials in the bovine diarrhea.
本研究描述了在印度健康牛（HC）和腹泻牛（DC）中，多重耐药（MDR）和产超广谱β-内酰胺酶（ESBL）肺炎克雷伯菌（KP）的发生率和特征比较。在 2018-2020 年期间，从 251 份直肠拭子（DC：219；HC：32）中检测了 72 株 MDR 分离株，包括 35 株（DC：27；HC：8）和 37 株（DC：34；HC：3），以研究其对扩展谱β-内酰胺酶（ESBL）、AmpC 型β-内酰胺酶和碳青霉烯酶的产生、抗菌药物敏感性谱、生物膜产生和外排泵活性。55 株 MDR 分离株为 ESBL 生产者（ESBLPs）（DC：50；HC：5），且 DC 中的 ESBLPs 对多种抗生素具有固有耐药性。CTX-M 基因（50）是最常检测到的β-内酰胺酶，其次是 AmpC（22）、TEM1（13）、CMY-6（6）、OXA1（5）、PER（2）、DHA 和 FOX 以及 SHV12（1）。在 18、16、2 和 3 株分离株中分别检测到质粒介导的喹诺酮耐药决定子、、、和。23 株分离株的 和 基因发生突变。在 33、10、3 和 2 株分离株中分别检测到四环素耐药标记物、、、和。在 41 株亚胺培南耐药分离株中只有 1 株携带 NDM-5，2 株对多粘菌素耐药。共有 20 株 MDR 分离株为强生物膜生产者，19 株携带不同的毒力因子。这是印度首次报告在牛腹泻中存在对甚至是最后一线抗菌药物都具有耐药性的多重耐药肠杆菌科细菌。
