Department of Engineering & Physics, University of Central Oklahoma, Edmond, OK, United States of America.
Department of Biology, University of Central Oklahoma, Edmond, OK, United States of America.
Biomed Mater. 2021 May 24;16(4). doi: 10.1088/1748-605X/abfd12.
Polyethylene glycol diacrylate (PEGDA) is an important class of photosensitive polymer with many tissue engineering applications. This study compared PEGDA and polycaprolactone (PCL) nanofiber matrix (NFM) coated PEGDA, referred to as PCL-PEGDA, scaffolds for their application in multiple tissue repair such as articular cartilage, nucleus pulposus of the intervertebral disc (IVD). We examined each scaffold morphology, porosity, swelling ratio, degradation, mechanical strength, andcytocompatibility properties. A defect was created in Sprague Dawley rat tail IVD by scraping native cartilage tissue and disc space, then implanting the scaffolds in the disc space for 4 weeks to evaluateefficacy of multi-tissue repair. Maintenance of disc height and creation of a new cell matrix was assessed to evaluate each scaffold's ability to repair the tissue defect. Although both PEGDA and PCL-PEGDA scaffolds showed similar porosity ∼73%, we observed distinct topographical characteristics and a higher effect of degradation on the water-absorbing capacity for PEGDA compared to PCL-PEGDA. Mechanical tests showed higher compressive strength and modulus of PCL-PEGDA compared to PEGDA.cell studies show that the PCL NFM layer covering PEGDA improved osteoblast cell adhesion, proliferation, and migration into the PEGDA layer.studies concluded that the PEGDA scaffold alone was not ideal for implantation in rat caudal disc space without PCL nanofiber coating due to low compressive strength and modulus.results confirm that the PCL-PEGDA scaffold-maintained disc space and created a proteoglycan and collagen-rich new tissue matrix in the defect site after 4 weeks of scaffold implantation. We concluded that our developed PCL-PEGDA has the potential to be used in multi-tissue defect site repair.
聚乙二醇二丙烯酸酯 (PEGDA) 是一类重要的光敏感聚合物，在许多组织工程应用中具有重要作用。本研究比较了 PEGDA 和聚己内酯 (PCL) 纳米纤维基质 (NFM) 涂层 PEGDA（简称 PCL-PEGDA）支架，用于关节软骨、椎间盘核（IVD）等多种组织的修复。我们检查了每种支架的形态、孔隙率、溶胀比、降解、机械强度和细胞相容性。通过刮除天然软骨组织和椎间盘间隙，在 Sprague Dawley 大鼠尾椎间盘中创建一个缺陷，然后将支架植入椎间盘间隙 4 周，以评估多组织修复的效果。评估椎间盘高度的维持和新细胞基质的形成，以评估每种支架修复组织缺陷的能力。尽管 PEGDA 和 PCL-PEGDA 支架的孔隙率相似（约 73%），但我们观察到 PEGDA 的形貌特征和降解对吸水性的影响明显不同。机械测试表明，PCL-PEGDA 的抗压强度和模量均高于 PEGDA。细胞研究表明，覆盖 PEGDA 的 PCL NFM 层提高了成骨细胞的黏附、增殖和向 PEGDA 层的迁移。研究得出结论，PEGDA 支架单独植入大鼠尾椎间盘空间不理想，因为没有 PCL 纳米纤维涂层，其抗压强度和模量较低。结果证实，PCL-PEGDA 支架在支架植入 4 周后维持了椎间盘间隙，并在缺陷部位形成了富含蛋白聚糖和胶原蛋白的新组织基质。我们得出结论，我们开发的 PCL-PEGDA 有可能用于多种组织缺陷部位的修复。
