Tan Sze-Jack, Lee Chee-Keong, Gan Chee-Yuen, Olalere Olusegun Abayomi
Main Campus, Tunku Abdul Rahman University College, Jalan Genting Klang, Kuala Lumpur 53300, Malaysia.
Bioprocess Division, School of Industrial Technology, Universiti Sains Malaysia, Gelugor 11800, Malaysia.
Molecules. 2021 Apr 1;26(7):2014. doi: 10.3390/molecules26072014.
In this study, the combination of parameters required for optimal extraction of anti-oxidative components from the Chinese lotus (CLR) and Malaysian lotus (MLR) roots were carefully investigated. Box-Behnken design was employed to optimize the pH (X: 2-3), extraction time (X: 0.5-1.5 h) and solvent-to-sample ratio (X: 20-40 mL/g) to obtain a high flavonoid yield with high % DPPH free radical scavenging and Ferric-reducing power assay (FRAP). The analysis of variance clearly showed the significant contribution of quadratic model for all responses. The optimal conditions for both Chinese lotus (CLR) and Malaysian lotus (MLR) roots were obtained as: CLR: X = 2.5; X = 0.5 h; X = 40 mL/g; MLR: X = 2.4; X = 0.5 h; X = 40 mL/g. These optimum conditions gave (a) Total flavonoid content (TFC) of 0.599 mg PCE/g sample and 0.549 mg PCE/g sample, respectively; (b) % DPPH of 48.36% and 29.11%, respectively; (c) FRAP value of 2.07 mM FeSO and 1.89 mM FeSO, respectively. A close agreement between predicted and experimental values was found. The result obtained succinctly revealed that the Chinese lotus exhibited higher antioxidant and total flavonoid content when compared with the Malaysia lotus root at optimum extraction condition.
在本研究中，对从中国莲藕（CLR）和马来西亚莲藕（MLR）根中最佳提取抗氧化成分所需的参数组合进行了仔细研究。采用Box-Behnken设计优化pH值（X：2 - 3）、提取时间（X：0.5 - 1.5小时）和溶剂与样品比例（X：20 - 40 mL/g），以获得高黄酮产量，并具有高% DPPH自由基清除率和铁还原能力测定（FRAP）。方差分析清楚地表明二次模型对所有响应的显著贡献。获得了中国莲藕（CLR）和马来西亚莲藕（MLR）根的最佳条件如下：CLR：X = 2.5；X = 0.5小时；X = 40 mL/g；MLR：X = 2.4；X = 0.5小时；X = 40 mL/g。这些最佳条件分别给出了（a）总黄酮含量（TFC）为0.599 mg PCE/g样品和0.549 mg PCE/g样品；（b）% DPPH分别为48.36%和29.11%；（c）FRAP值分别为2.07 mM FeSO和1.89 mM FeSO。发现预测值与实验值之间有密切的一致性。所得结果简洁地表明，在最佳提取条件下，中国莲藕与马来西亚莲藕根相比表现出更高的抗氧化剂和总黄酮含量。
