Department of Pharmacognosy, Poznan University of Medical Sciences, Rokietnicka 3, 60-806 Poznan, Poland.
Faculty of Chemistry, Adam Mickiewicz University in Poznań, Uniwersytetu Poznańskiego 8, 61-614 Poznan, Poland.
Molecules. 2022 Oct 5;27(19):6616. doi: 10.3390/molecules27196616.
Herbal raw materials with antidiabetic activity can be a valuable support to therapy. An optimized extraction process allows for the best possible health-promoting effect. Box-Behnken design was employed to optimize the content of methanol used in the extraction mixture, its time, and temperature. The aim of this study was to enhance the efficiency of the pomegranate flowers extraction process in order to obtain extracts with the highest enzyme inhibition power (α-amylase and α-glucosidase), which is important for the antidiabetic effect and the highest antioxidant activity (DPPH assay). In the Box-Behnken design model, the content of pelargonidin-3,5-glucoside-anthocyanin compound that is associated with antidiabetic activity was also optimized as a variable associated with the action profile of pomegranate flower extracts. The process optimization carried out in this study provides a basis for further research using the pomegranate flower extract with the most potent desired properties, essential for supporting diabetes treatment based on pomegranate flowers.
具有降血糖活性的草药原料可以成为治疗的有价值的辅助手段。优化的提取工艺可以实现尽可能好的促进健康的效果。采用 Box-Behnken 设计来优化甲醇在提取混合物中的用量、时间和温度。本研究的目的是提高石榴花提取过程的效率，以获得具有最高酶抑制能力（α-淀粉酶和 α-葡萄糖苷酶）的提取物，这对于降血糖作用和最高抗氧化活性（DPPH 测定）很重要。在 Box-Behnken 设计模型中，还优化了与降血糖活性相关的矢车菊素-3,5-葡萄糖苷-花色苷化合物的含量，作为与石榴花提取物作用谱相关的变量。本研究中的工艺优化为使用具有最强所需特性的石榴花提取物进行进一步研究提供了基础，这对于基于石榴花的糖尿病治疗支持是必不可少的。
