Tokumine Y, Ueda E, Ogawa H, Sugiyama H, Taniwaki M, Abe T, Kanayama Y, Hashimoto T, Inoue R, Machii T
Department of Clinical Research, Osaka University, Japan.
Int J Cancer. 1988 Jul 15;42(1):99-103. doi: 10.1002/ijc.2910420119.
A hairy-cell leukemia (HCL) line, BNBH-I, was established from the peripheral blood of a 40-year-old male patient with HCL in a relatively stable clinical phase after splenectomy. The cells have since been growing continuously for more than 2 years. Their cell surface immunoglobulin (sIg) was identical with that found on the surface of freshly isolated leukemic cells, consisting of IgG-kappa. The BNBH-I cells were more mature than the original hairy cells in their degree of B-cell differentiation, as reflected by a decrease in sIg expression together with the appearance of some cytoplasmic Ig (cIg)+ cells, loss of EA gamma-rosette formation and reactivity with monoclonal antibody (MAb) FMC7, and an increase in the proportion of MAb PCA-I+ cells. The BNBH-I cells possessed the antigen recognized by Leu-M5, a highly specific MAb for HCL. Epstein-Barr virus nuclear antigen (EBNA) was present. Both the freshly isolated leukemic cells and the cell line had the 14q+ involving q32 chromosomal abnormality, and their Ig gene rearrangements were also identical. Following exposure to 12-O-tetradecanoylphorbol-13-acetate (TPA), both the freshly isolated leukemic cells and the BNBH-I cells adhered to culture dishes and extended long, thin processes, a phenomenon characteristic of HCL. These results indicate that the BNBH-I line was derived from the leukemic hairy cells.
一株毛细胞白血病（HCL）细胞系BNBH-I，是从一名40岁男性HCL患者脾切除术后处于相对稳定临床阶段的外周血中建立的。自那时起，这些细胞已连续生长超过2年。它们的细胞表面免疫球蛋白（sIg）与新鲜分离的白血病细胞表面发现的相同，由IgG-κ组成。BNBH-I细胞在B细胞分化程度上比原始毛细胞更成熟，这表现为sIg表达减少，同时出现一些细胞质Ig（cIg）+细胞，EAγ-玫瑰花结形成丧失以及与单克隆抗体（MAb）FMC7的反应性丧失，并且MAb PCA-I+细胞比例增加。BNBH-I细胞具有被Leu-M5识别的抗原，Leu-M5是一种针对HCL的高度特异性单克隆抗体。存在爱泼斯坦-巴尔病毒核抗原（EBNA）。新鲜分离的白血病细胞和细胞系均有涉及q32的14q+染色体异常，并且它们的Ig基因重排也相同。暴露于12-O-十四酰佛波醇-13-乙酸酯（TPA）后，新鲜分离的白血病细胞和BNBH-I细胞均黏附于培养皿并伸出长而细的突起，这是HCL的特征性现象。这些结果表明BNBH-I细胞系源自白血病毛细胞。
